Amphiphilic graft copolymer based on poly(styrene-co-maleic anhydride) with low molecular weight polyethylenimine for efficient gene delivery
文献类型：期刊论文
| 作者 | Duan, Xiaopin1,2; Xiao, Jisheng1; Yin, Qi1 ; Zhang, Zhiwen1; Mao, Shirui2; Li, Yaping1
|
| 刊名 | INTERNATIONAL JOURNAL OF NANOMEDICINE
 |
| 出版日期 | 2012 |
| 卷号 | 7页码:4961-4972 |
| 关键词 | poly(styrene-co-maleic anhydride) polyethylenimine DNA gene delivery |
| ISSN号 | 1178-2013 |
| DOI | 10.2147/IJN.S32069 |
| 文献子类 | Article |
| 英文摘要 | Background and methods: A new amphiphilic comb-shaped copolymer (SP) was synthesized by conjugating poly(styrene-co-maleic anhydride) with low molecular weight polyethyleneimine for gene delivery. Fourier transform infrared spectrum, H-1 nuclear magnetic resonance, and gel permeation chromatography were used to characterize the graft copolymer. Results: The buffering capability of SP was similar to that of polyethyleneimine within the endosomal pH range. The copolymer could condense DNA effectively to form complexes with a positive charge (13-30 mV) and a small particle size (130-200 nm) at N/P ratios between 5 and 20, and protect DNA from degradation by DNase I. In addition, SP showed much lower cytotoxicity than polyethyleneimine 25,000. Importantly, the gene transfection activity and cellular uptake of SP-DNA complexes were all markedly higher than that of complexes of polyethyleneimine 25,000 and DNA in MCF-7 and MCF-7/ADR cell lines. Conclusion: This work highlights the promise of SP as a safe and efficient synthetic vector for DNA delivery. |
| WOS关键词 | IN-VIVO ; THERAPY ; VECTOR ; CANCER ; DERIVATIVES ; DESIGN ; ACID) ; DRUG ; NANOPARTICLES ; TRANSFECTION |
| 资助项目 | National Basic Research Program of China[2012CB932502] ; National Basic Research Program of China[2010CB934000] ; National Natural Science Foundation of China[30901866] ; National Natural Science Foundation of China[30925041] ; Shanghai Nanomedicine Program[11nm0505900] ; Shanghai Rising Star Program[11QA1407900] ; Shanghai Outstanding Academic Leaders Program[11XD1406200] |
| WOS研究方向 | Science & Technology - Other Topics ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000308699200001 |
| 出版者 | DOVE MEDICAL PRESS LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278242]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Li, Yaping |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Pharmaceut, Shanghai 200031, Peoples R China 2.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Duan, Xiaopin,Xiao, Jisheng,Yin, Qi,et al. Amphiphilic graft copolymer based on poly(styrene-co-maleic anhydride) with low molecular weight polyethylenimine for efficient gene delivery[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2012,7:4961-4972. |
| APA | Duan, Xiaopin,Xiao, Jisheng,Yin, Qi,Zhang, Zhiwen,Mao, Shirui,&Li, Yaping.(2012).Amphiphilic graft copolymer based on poly(styrene-co-maleic anhydride) with low molecular weight polyethylenimine for efficient gene delivery.INTERNATIONAL JOURNAL OF NANOMEDICINE,7,4961-4972. |
| MLA | Duan, Xiaopin,et al."Amphiphilic graft copolymer based on poly(styrene-co-maleic anhydride) with low molecular weight polyethylenimine for efficient gene delivery".INTERNATIONAL JOURNAL OF NANOMEDICINE 7(2012):4961-4972. |
入库方式： OAI收割
来源：上海药物研究所
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