Skin-permeable quaternary nanoparticles with layer-by-layer structure enabling improved gene delivery
文献类型：期刊论文
| 作者 | Yang, Yongxin; Jiang, Yifan; Wang, Zhao; Liu, Jinhua; Yan, Lu; Ye, Junxiao; Huang, Yongzhuo
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| 刊名 | JOURNAL OF MATERIALS CHEMISTRY
 |
| 出版日期 | 2012 |
| 卷号 | 22期号:19页码:10029-10034 |
| ISSN号 | 0959-9428 |
| DOI | 10.1039/c2jm00121g |
| 文献子类 | Article |
| 英文摘要 | Skin is an attractive target for delivery of genetic therapeutics and vaccines. However, the effective delivery of nucleic acids through the skin is primarily limited by the highly organized structure of the stratum corneum. Herein we propose a novel strategy to overcome the formidable barrier of skin by using the skin-permeable LMWP/PGA/PEI/DNA quaternary nanoparticles (NPs) with a self-assembling layer-by-layer structure. The ternary layer of anionic poly(g-glutamic acid) (PGA) coats the cationic nano-core of PEI/DNA, and the outermost layer is the cell-penetrating low molecular weight protamine (LMWP) peptide. The LMWP functions as a biological permeation enhancer, promoting not only skin permeation but also cell penetration. Our results showed that the cellular uptake and transfection level were greatly improved by the LMWP-modified quaternary NPs compared with the conventional PEI/DNA NPs, displaying a four-fold increase. Skin penetration of the quaternary NPs was significant, showing the high cumulative amount of percutaneous delivery up to 14% of the applied dose. Importantly, skin penetration efficiency of the quaternary NPs was further enhanced by a synergistic effect of LMWP and the chemical permeation enhancer sodium lauryl sulfate up to 23.8%. The results suggested that the skin-permeable NPs could be a promising approach for percutaneous gene delivery. |
| WOS关键词 | CELL-PENETRATING PEPTIDES ; MOLECULAR-WEIGHT PROTAMINE ; TRANSDERMAL DRUG-DELIVERY ; PROTEIN TRANSDUCTION ; ABSORPTION ; DOMAINS |
| 资助项目 | National Natural Science Foundation of China (NSFC)[91029743] ; National Natural Science Foundation of China (NSFC)[81172996] ; National Natural Science Foundation of China (NSFC)[81072231] ; Shanghai Pu-jiang Scholar Program[00000000] |
| WOS研究方向 | Chemistry ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000303207100085 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278262]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Huang, Yongzhuo |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yongxin,Jiang, Yifan,Wang, Zhao,et al. Skin-permeable quaternary nanoparticles with layer-by-layer structure enabling improved gene delivery[J]. JOURNAL OF MATERIALS CHEMISTRY,2012,22(19):10029-10034. |
| APA | Yang, Yongxin.,Jiang, Yifan.,Wang, Zhao.,Liu, Jinhua.,Yan, Lu.,...&Huang, Yongzhuo.(2012).Skin-permeable quaternary nanoparticles with layer-by-layer structure enabling improved gene delivery.JOURNAL OF MATERIALS CHEMISTRY,22(19),10029-10034. |
| MLA | Yang, Yongxin,et al."Skin-permeable quaternary nanoparticles with layer-by-layer structure enabling improved gene delivery".JOURNAL OF MATERIALS CHEMISTRY 22.19(2012):10029-10034. |
入库方式： OAI收割
来源：上海药物研究所
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