Bioreducible poly (beta-amino esters)/shRNA complex nanoparticles for efficient RNA delivery
文献类型：期刊论文
| 作者 | Yin, Qi ; Gao, Yu; Zhang, Zhiwen; Zhang, Pengcheng; Li, Yaping
|
| 刊名 | JOURNAL OF CONTROLLED RELEASE
 |
| 出版日期 | 2011-04-10 |
| 卷号 | 151期号:1页码:35-44 |
| 关键词 | Poly (beta-amine esters) Bioreducible Non-viral vector RNA delivery Nanoparticles |
| ISSN号 | 0168-3659 |
| DOI | 10.1016/j.jconrel.2010.12.014 |
| 文献子类 | Article |
| 英文摘要 | RNA interference (RNAi) mediating gene silencing is a promising approach in the area of gene therapy, but it still is a major challenge to find new non-viral vectors with high transfection efficiency and low toxicity until today. In this work, three novel bioreducible poly (beta-amine esters) (PAEs) with different amino monomers in the main chain were designed and synthesized by Michael addition polymerization. All PAEs could condense shRNA into complex nanoparticles with particle size (60-200 nm) and positive surface charges (>+10 mV). The PAEs/shRNA complex nanoparticles (PAENs) were stable under the extracellular physiological condition, while it would degrade in the reductive environment due to the cleavage of the disulfide bonds in the PAEs main chain. PAENs could achieve efficient cellular uptake and EGFP silencing in HEK-293 cells and U-87 MG cells with low cytotoxicity. The high accumulation of PAENs in tumor and high silencing efficiency of intratumor EGFP expression occurred when PAENs were intravenously injected into BALB/c mice bearing U-87 MG-GFP tumor. The relationship between the polymer structure and RNAi efficiency and cytotoxicity showed that the density of nitrogen atoms in PAEs backbone and the existence of disulfide bonds demonstrated the remarkable influence on in vitro and in vivo gene silencing efficiency and cytotoxicity. These experimental results suggested that the PAENs could be a promising non-viral vector for efficient RNA delivery. (C) 2011 Elsevier B.V. All rights reserved. |
| WOS关键词 | GENE DELIVERY ; IN-VIVO ; SIRNA ; SYSTEM ; TRANSFECTION ; CHITOSAN ; VECTOR ; CELLS ; DNA |
| 资助项目 | National Basic Research Program of China[2010CB934000] ; National Basic Research Program of China[2007CB935800] ; National Natural Science Foundation of China[30925041] ; National Natural Science Foundation of China[30873169] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program"[2009ZX09501-024] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program"[2009ZX09301-001] ; Shanghai Nanomedicine Program[1052nm06300] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000291240900006 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278558]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Li, Yaping |
| 作者单位 | Chinese Acad Sci, Ctr Pharmaceut, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Qi,Gao, Yu,Zhang, Zhiwen,et al. Bioreducible poly (beta-amino esters)/shRNA complex nanoparticles for efficient RNA delivery[J]. JOURNAL OF CONTROLLED RELEASE,2011,151(1):35-44. |
| APA | Yin, Qi,Gao, Yu,Zhang, Zhiwen,Zhang, Pengcheng,&Li, Yaping.(2011).Bioreducible poly (beta-amino esters)/shRNA complex nanoparticles for efficient RNA delivery.JOURNAL OF CONTROLLED RELEASE,151(1),35-44. |
| MLA | Yin, Qi,et al."Bioreducible poly (beta-amino esters)/shRNA complex nanoparticles for efficient RNA delivery".JOURNAL OF CONTROLLED RELEASE 151.1(2011):35-44. |
入库方式： OAI收割
来源：上海药物研究所
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