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Polysorbate cationic synthetic vesicle for gene delivery

文献类型：期刊论文

作者Huang, Yongzhuo2,3; Rao, Yuefeng1,4; Chen, Jinliang1,4; Yang, Victor C.2; Liang, Wenquan1,4
刊名JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
出版日期2011-03-01
卷号96A期号:3页码:513-519
关键词nonionic surfactant synthetic vesicle noisome polysorbates gene delivery
ISSN号1549-3296
DOI10.1002/jbm.a.32999
文献子类Article
英文摘要Synthetic nonionic surfactant vesicles (niosomes) are a colloidal system with closed bilayer structures, displaying distinct advantages in stability and cost compared with liposomes. In this article, polysorbate cationic niosomes (PCNs) were developed as gene carriers. The PCNs comprised nonionic surfactants (i.e., polysorbates) and a cationic cholesterol, and were synthesized using a film hydration method. The niosomes thus prepared possessed a regular morphology, and a particle size of 100 similar to 200 nm, and a zeta potential of +30 similar to 45 mV. The PCNs showed great physical stability over the course of 4 weeks at room temperature. The binding capacity of PCNs toward oligo-deoxynucleotides (ODN) was assessed by a gel retardation approach, which demonstrated that the ionic complexes were formed when +/- charge ratio reached to 4 or greater. Gene transfer study showed that the PCNs exhibited a high efficiency in mediating cellular uptake and transferred DNA expression. Based on these findings, PCNs may offer the potential to function as an effective gene delivery system. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 96A: 513-519, 2011.
WOS关键词IN-VITRO ; NONIONIC SURFACTANTS ; SORBITAN MONOESTER ; ORAL DELIVERY ; NIOSOMES ; EMULSION ; TRANSFECTION ; LIPOSOME ; INSULIN ; CELLS
资助项目NIH[CA114612] ; NIH[NS066945] ; WCU project of South Korea[R31-2008-000-10103-01] ; Chinese Academy of Sciences[00000000]
WOS研究方向Engineering ; Materials Science
语种英语
WOS记录号WOS:000286953000002
出版者WILEY
源URL[http://119.78.100.183/handle/2S10ELR8/278595]  
专题药物制剂研究中心
通讯作者Yang, Victor C.
作者单位1.Zhejiang Univ, Coll Med, Affiliated Hosp 1, Hangzhou 310058, Zhejiang, Peoples R China;
2.Univ Michigan, Coll Pharm, Ann Arbor, MI 48109 USA;
3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
4.Zhejiang Univ, Coll Pharmaceut Sci, Hangzhou 310058, Zhejiang, Peoples R China
推荐引用方式
GB/T 7714		 Huang, Yongzhuo,Rao, Yuefeng,Chen, Jinliang,et al. Polysorbate cationic synthetic vesicle for gene delivery[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2011,96A(3):513-519.
APA Huang, Yongzhuo,Rao, Yuefeng,Chen, Jinliang,Yang, Victor C.,&Liang, Wenquan.(2011).Polysorbate cationic synthetic vesicle for gene delivery.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,96A(3),513-519.
MLA Huang, Yongzhuo,et al."Polysorbate cationic synthetic vesicle for gene delivery".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 96A.3(2011):513-519.

入库方式： OAI收割

来源：上海药物研究所

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