Downregulation of survivin expression and enhanced chemosensitivity of MCF-7 cells to adriamycin by PDMAE/survivin shRNA complex nanoparticles
文献类型：期刊论文
| 作者 | Yang, Yongxin; Gao, Yu; Chen, Lingli ; Huang, Yongzhuo; Li, Yaping
|
| 刊名 | INTERNATIONAL JOURNAL OF PHARMACEUTICS
 |
| 出版日期 | 2011-02-28 |
| 卷号 | 405期号:1-2页码:188-195 |
| 关键词 | RNAi shRNA Adriamycin Survivin Poly(2-dimethylaminoethylamine/2-(2-aminoethyoxy)ethoxy)phosphazene (PDMAE) |
| ISSN号 | 0378-5173 |
| DOI | 10.1016/j.ijpharm.2010.11.047 |
| 文献子类 | Article |
| 英文摘要 | Gene silencing mediated by RNA interference (RNAi) presents a promising strategy for gene therapy. The aim of this work is to evaluate a new gene delivery system for downregulation of survivin expression and enhanced chemosensitivity of MCF-7 cells to adriamycin (ADR). A new cationic poly(2-dimethylaminoethylamine/2-(2-aminoethyoxy)ethoxy)phosphazene (PDMAE) with multiple amino groups was synthesized through Michael addition for survivin shRNA (shSur) delivery in MCF-7 cells. PDMAE51/shSur complex nanoparticles with the size of 190 nm and zeta potential of +15 mV achieved maximal suppression of survivin, even superior to PEI25K or poly(2-(2-aminoethyoxy)ethoxy)phosphazene (PAEP) based complex nanoparticles. The significant downregulation of survivin expression was achieved by PDMAE51/shSur nanoparticles. The nuclear localization by confocal laser scanning microscopy (CLSM) and apparent apoptosis peak of cell cycle in MCF-7 cells were observed when transfected by PDMAE51/shSur nanoparticles The combined use of PDMAE51/shSur and ADR enhanced the sensitivity of MCF-7 cells to ADR at a larger extent than that of PEI or PAEP based complex nanoparticles. These results suggested that PDMAE51 could be potential as an efficient and safe gene carrier in RNAi therapeutics and tumor chemotherapy. (C) 2010 Elsevier B.V. All rights reserved. |
| WOS关键词 | GENE DELIVERY ; LUNG-CANCER ; TARGETING SURVIVIN ; RNA INTERFERENCE ; IN-VITRO ; SIRNA ; TRANSFECTION ; NUCLEAR ; CARRIER |
| 资助项目 | National Basic Research Program of China[2010CB934000] ; National Basic Research Program of China[2007CB935800] ; National Natural Science Foundation of China[30925041] ; National Natural Science Foundation of China[30901866] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program"[2009ZX09501-024] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program"[2009ZX09301-001] ; Shanghai Nanomedicine Program[1052nm06300] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000287279400022 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278597]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Huang, Yongzhuo |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Pharmaceut, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yongxin,Gao, Yu,Chen, Lingli,et al. Downregulation of survivin expression and enhanced chemosensitivity of MCF-7 cells to adriamycin by PDMAE/survivin shRNA complex nanoparticles[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2011,405(1-2):188-195. |
| APA | Yang, Yongxin,Gao, Yu,Chen, Lingli,Huang, Yongzhuo,&Li, Yaping.(2011).Downregulation of survivin expression and enhanced chemosensitivity of MCF-7 cells to adriamycin by PDMAE/survivin shRNA complex nanoparticles.INTERNATIONAL JOURNAL OF PHARMACEUTICS,405(1-2),188-195. |
| MLA | Yang, Yongxin,et al."Downregulation of survivin expression and enhanced chemosensitivity of MCF-7 cells to adriamycin by PDMAE/survivin shRNA complex nanoparticles".INTERNATIONAL JOURNAL OF PHARMACEUTICS 405.1-2(2011):188-195. |
入库方式： OAI收割
来源：上海药物研究所
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