Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism
文献类型：期刊论文
| 作者 | Zhang, Zhiwen1,2,3 ; Fang, Xiaoling2,3; Hao, Junguo2,3; Li, Yajuan2,3; Sha, Xianyi2,3
|
| 刊名 | INTERNATIONAL JOURNAL OF NANOMEDICINE
 |
| 出版日期 | 2011 |
| 卷号 | 6页码:2235-2244 |
| 关键词 | triolein dioleoyl phosphatidylethanolalmine polyethylenimine lipid nanocarrier mechanism |
| ISSN号 | 1178-2013 |
| DOI | 10.2147/IJN.S24720 |
| 文献子类 | Article |
| 英文摘要 | We proposed to develop a polycation lipid nanocarrier (PLN) with higher - transfection efficiency than our previously described polycation nanostrucutred lipid nanocarrier (PNLC). PLN was composed of triolein, cetylated low-molecular-weight polyethylenimine, and dioleoyl phosphatidylethanolamine. The physicochemical properties of PLN and the PLN/DNA complexes (PDC) were characterized. The in vitro transfection was performed in human lung adenocarcinoma (SPC-A1) cells, and the intracellular mechanism was investigated as well. The measurements indicated that PLN and PDC are homogenous nanometer-sized particles with a positive charge. The transfection efficiency of PDC significantly increased with the content of triolein and was higher than that of PNLC and commercial Lipofectamine (TM) 2000. In particular, the transfection of PLN in the presence of 10% serum was more effective than that in its absence. With the help of specific inhibitors of chlorpromazine and filipin, the clathrin-dependent endocytosis pathway was determined to be the main contributor to the successful transfection mediated by PLN in SPC-A1 cells. The captured images verified that the fluorescent PDC was localized in the lysosomes and nuclei after endocytosis. Thus, PLN represents a novel efficient nonviral gene delivery vector. |
| WOS关键词 | NONVIRAL VECTOR ; TRANSFER SYSTEM ; DNA VACCINE ; POLYETHYLENIMINE ; LIPOSOMES ; TRANSFECTION ; COMPLEXES ; ENDOCYTOSIS ; EXPRESSION ; TOXICITY |
| 资助项目 | National Natural Science Foundation of China[30901862] ; National Natural Science Foundation of China[30901866] ; National Natural Science Foundation of China[30873177] ; Shanghai Rising-Star Program[10QA1400800] ; Shanghai Rising-Star Program[11QA1407900] ; Scientific Research Foundation for the Returned Overseas Chinese Scholar[00000000] |
| WOS研究方向 | Science & Technology - Other Topics ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000297676300001 |
| 出版者 | DOVE MEDICAL PRESS LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278648]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Sha, Xianyi |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China 2.Fudan Univ, Dept Pharmaceut, Sch Pharm, Key Lab Smart Drug Delivery,Minist Educ, Shanghai 201203, Peoples R China; 3.Fudan Univ, PLA, Sch Pharm, Dept Pharmaceut, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhang, Zhiwen,Fang, Xiaoling,Hao, Junguo,et al. Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2011,6:2235-2244. |
| APA | Zhang, Zhiwen,Fang, Xiaoling,Hao, Junguo,Li, Yajuan,&Sha, Xianyi.(2011).Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism.INTERNATIONAL JOURNAL OF NANOMEDICINE,6,2235-2244. |
| MLA | Zhang, Zhiwen,et al."Triolein-based polycation lipid nanocarrier for efficient gene delivery: characteristics and mechanism".INTERNATIONAL JOURNAL OF NANOMEDICINE 6(2011):2235-2244. |
入库方式： OAI收割
来源：上海药物研究所
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