Novel mucus-penetrating liposomes as a potential oral drug delivery system: preparation, in vitro characterization, and enhanced cellular uptake
文献类型：期刊论文
| 作者 | Li, Xiuying4; Chen, Dan4; Le, Chaoyi3; Zhu, Chunliu4 ; Gan, Yong4; Hovgaard, Lars2; Yang, Mingshi1
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| 刊名 | INTERNATIONAL JOURNAL OF NANOMEDICINE
 |
| 出版日期 | 2011 |
| 卷号 | 6页码:3151-3162 |
| 关键词 | Pluronic F127 mucus-penetrating particles liposomes oral drug delivery |
| ISSN号 | 1178-2013 |
| DOI | 10.2147/IJN.S25741 |
| 文献子类 | Article |
| 英文摘要 | Background: The aim of this study was to investigate the intestinal mucus-penetrating properties and intestinal cellular uptake of two types of liposomes modified by Pluronic F127 (PF127). Methods: The two types of liposomes, ie, PF127-inlaid liposomes and PF127-adsorbed liposomes, were prepared by a thin-film hydration method followed by extrusion, in which coumarin 6 was loaded as a fluorescence marker. A modified Franz diffusion cell mounted with the intestinal mucus of rats was used to study the diffusion characteristics of the two types of PF127 liposomes. Cell uptake studies were conducted in Caco-2 cells and analyzed using confocal laser scanning microcopy as well as flow cytometry. Results: The diffusion efficiency of the two types of PF127-modified liposomes through intestinal rat mucus was 5-7-fold higher than that of unmodified liposomes. Compared with unmodified liposomes, PF127-inlaid liposomes showed significantly higher cellular uptake of courmarin 6. PF127-adsorbed liposomes showed a lower cellular uptake. Moreover, and interestingly, the two types of PF127-modified liposomes showed different cellular uptake mechanisms in Caco-2 cells. Conclusion: PF127-inlaid liposomes with improved intestinal mucus-penetrating ability and enhanced cellular uptake might be a potential carrier candidate for oral drug delivery. |
| WOS关键词 | GASTROINTESTINAL MUCUS ; PLGA-POLOXAMER ; NANOPARTICLES ; TRANSPORT ; SPUTUM ; VIVO ; MONOLAYERS ; VESICLES ; BARRIER ; FATE |
| 资助项目 | Novo Nordisk-Chinese Academy of Science Research Foundation[NNCAS-2009-10] ; National Science and Technology Major Project Key New Drug Creation and Manufacturing Program[2009ZX09301-001] ; National Basic Research Program of China[2009CB930300] |
| WOS研究方向 | Science & Technology - Other Topics ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000297694700001 |
| 出版者 | DOVE MEDICAL PRESS LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278649]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Gan, Yong |
| 作者单位 | 1.Univ Copenhagen, Dept Pharmaceut & Analyt Chem, Copenhagen, Denmark 2.Novo Nordisk AS, Oral Formulat Dev, Maalov, Russia; 3.Univ Toronto, Toronto, ON M5S 1A1, Canada; 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Li, Xiuying,Chen, Dan,Le, Chaoyi,et al. Novel mucus-penetrating liposomes as a potential oral drug delivery system: preparation, in vitro characterization, and enhanced cellular uptake[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2011,6:3151-3162. |
| APA | Li, Xiuying.,Chen, Dan.,Le, Chaoyi.,Zhu, Chunliu.,Gan, Yong.,...&Yang, Mingshi.(2011).Novel mucus-penetrating liposomes as a potential oral drug delivery system: preparation, in vitro characterization, and enhanced cellular uptake.INTERNATIONAL JOURNAL OF NANOMEDICINE,6,3151-3162. |
| MLA | Li, Xiuying,et al."Novel mucus-penetrating liposomes as a potential oral drug delivery system: preparation, in vitro characterization, and enhanced cellular uptake".INTERNATIONAL JOURNAL OF NANOMEDICINE 6(2011):3151-3162. |
入库方式： OAI收割
来源：上海药物研究所
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