Linear Cationic Click Polymer for Gene Delivery: Synthesis, Biocompatibility, and In Vitro Transfection
文献类型：期刊论文
| 作者 | Gao, Yu; Chen, Lingli ; Zhang, Zhiwen; Gu, Wangwen; Li, Yaping
|
| 刊名 | BIOMACROMOLECULES
 |
| 出版日期 | 2010-11 |
| 卷号 | 11期号:11页码:3102-3111 |
| ISSN号 | 1525-7797 |
| DOI | 10.1021/bm100906m |
| 文献子类 | Article |
| 英文摘要 | Sixteen novel cationic click polymers (CPs) were parallelly synthesized via the conjugation of our alkyne-functionalized monomers to four azide-functionalized monomers by "click chemistry". The biocompatibility of CPs was evaluated by in vitro cytotoxicity (MTT assay, Hoechst/PI apoptosis/necrosis assay, and cell cycle analysis) and blood compatibility tests (hemolysis and erythrocyte aggregation). The experimental results showed that the kind of amine groups, charge density, and number of methylene or ethylene glycol groups brought about the effect on toxicity of CPs. Among all polymers, two polymers (B-1 and B-2) showed good biocompatibility. inducing neither apoptosis nor necrosis at the test concentration and low hemolysis ratio and erythrocyte aggregation. In particular, B-1 and B-2 exhibited the comparable transfection efficiency compared with PEI (25 kDa) but much lower cytotoxicity. These results suggested that the novel cationic CPs could be promising carriers for gene delivery. |
| WOS关键词 | PARALLEL SYNTHESIS ; PLASMID DNA ; CELL-DEATH ; CHEMISTRY ; APOPTOSIS ; CYCLOADDITION ; FUNCTIONALITY ; AGGREGATION ; POLYPLEXES ; COPOLYMERS |
| 资助项目 | National Basic Research Program of China[2010CB934000] ; National Basic Research Program of China[2007CB935800] ; National Natural Science Foundation of China[30925041] ; National Natural Science Foundation of China[30901866] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program"[2009ZX09501-024] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program"[2009ZX09301-001] ; Shanghai Nanomedicine Program[0852 nm05700] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000283810900036 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278729]  |
| 专题 | 药物制剂研究中心 上海中药现代化研究中心 |
| 通讯作者 | Li, Yaping |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Pharmaceut, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Yu,Chen, Lingli,Zhang, Zhiwen,et al. Linear Cationic Click Polymer for Gene Delivery: Synthesis, Biocompatibility, and In Vitro Transfection[J]. BIOMACROMOLECULES,2010,11(11):3102-3111. |
| APA | Gao, Yu,Chen, Lingli,Zhang, Zhiwen,Gu, Wangwen,&Li, Yaping.(2010).Linear Cationic Click Polymer for Gene Delivery: Synthesis, Biocompatibility, and In Vitro Transfection.BIOMACROMOLECULES,11(11),3102-3111. |
| MLA | Gao, Yu,et al."Linear Cationic Click Polymer for Gene Delivery: Synthesis, Biocompatibility, and In Vitro Transfection".BIOMACROMOLECULES 11.11(2010):3102-3111. |
入库方式： OAI收割
来源：上海药物研究所
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