Folate-PEG modified poly(2-(2-aminoethoxy)ethoxy)phosphazene/DNA nanoparticles for gene delivery: Synthesis, preparation and in vitro transfection efficiency
文献类型：期刊论文
| 作者 | Zhang, Pengcheng ; Zhang, Zhiwen; Yang, Yongxin; Li, Yaping
|
| 刊名 | INTERNATIONAL JOURNAL OF PHARMACEUTICS
 |
| 出版日期 | 2010-06-15 |
| 卷号 | 392期号:1-2页码:241-248 |
| 关键词 | DNA Poly(phosphazene) Folic acid Nanoparticles Gene |
| ISSN号 | 0378-5173 |
| DOI | 10.1016/j.ijpharm.2010.03.030 |
| 文献子类 | Article |
| 英文摘要 | Target-specific technique can significantly enhance the efficacy of gene delivery system which was limited by many cellular barriers. In this work, a new folate-PEG modified poly(2-(2-aminoethoxy)ethoxy)phosphazene (PAEP), namely, folate-PEG-PAEP was synthesized as a folate receptor (FR) targeted carrier, and the cytotoxicity, transfection efficiency, cellular uptake and intracellular trafficking of folate-PEG-PAEP/DNA nanoparticles (FPPN) were investigated. Compared with the PAEP/DNA nanoparticles (PN), the cytotoxicity of FPPN decreased significantly at high dose. FPPN showed much higher transfection efficiency (15.85 +/- 1.23%) compared with PN (6.71 +/- 0.42%) in FR overexpressing Hela cells, but no significant difference was observed in CHO-k1 cells lacking FR. The transfection activity of FPPN could be reversed in the presence of 1.0 mM free folic acid in Hela cells. The cellular uptake of FPPN was 37.38% higher than that of PN in Hela cells. These results indicated that FPPN could be a potential targeted gene delivery system. (C) 2010 Elsevier B.V. All rights reserved. |
| WOS关键词 | SELF-ASSEMBLED NANOPARTICLES ; POLY(ETHYLENE GLYCOL) ; MOLECULAR-WEIGHT ; NUCLEIC-ACIDS ; TUMOR ; CELLS ; DNA ; CYTOTOXICITY ; COPOLYMERS ; CARRIERS |
| 资助项目 | National Basic Research Program of China[2010CB934000] ; National Basic Research Program of China[2007CB935800] ; National Natural Science Foundation of China[30925041] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program"[2009ZX09501-024] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program"[2009ZX09301-001] ; Shanghai Nanomedicine Program[0852nm05700] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000278346500033 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278873]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Li, Yaping |
| 作者单位 | Chinese Acad Sci, Ctr Drug Delivery Syst, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Pengcheng,Zhang, Zhiwen,Yang, Yongxin,et al. Folate-PEG modified poly(2-(2-aminoethoxy)ethoxy)phosphazene/DNA nanoparticles for gene delivery: Synthesis, preparation and in vitro transfection efficiency[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2010,392(1-2):241-248. |
| APA | Zhang, Pengcheng,Zhang, Zhiwen,Yang, Yongxin,&Li, Yaping.(2010).Folate-PEG modified poly(2-(2-aminoethoxy)ethoxy)phosphazene/DNA nanoparticles for gene delivery: Synthesis, preparation and in vitro transfection efficiency.INTERNATIONAL JOURNAL OF PHARMACEUTICS,392(1-2),241-248. |
| MLA | Zhang, Pengcheng,et al."Folate-PEG modified poly(2-(2-aminoethoxy)ethoxy)phosphazene/DNA nanoparticles for gene delivery: Synthesis, preparation and in vitro transfection efficiency".INTERNATIONAL JOURNAL OF PHARMACEUTICS 392.1-2(2010):241-248. |
入库方式： OAI收割
来源：上海药物研究所
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