Effect of multifold charge groups and imidazole-4-carboxaldehyde on physicochemical characteristics and transfection of cationic polyphosphazenes/DNA complexes
文献类型：期刊论文
| 作者 | Yang, Yongxin; Zhang, Zhiwen ; Chen, Lingli; Li, Yaping
|
| 刊名 | INTERNATIONAL JOURNAL OF PHARMACEUTICS
 |
| 出版日期 | 2010-05-10 |
| 卷号 | 390期号:2页码:191-197 |
| 关键词 | Poly(2-(2-aminoethyoxy)ethoxy)phosphazene DNA Imidazole-4-carboxaldehyde Gene delivery Complexes |
| ISSN号 | 0378-5173 |
| DOI | 10.1016/j.ijpharm.2010.01.005 |
| 文献子类 | Article |
| 英文摘要 | To understand the dual influence of multifold charge groups and conjugation of imidazole moiety on the physicochemical characteristics and the transfection activity of polymer complexes, a series of cationic polyphosphazenes based on poly(2-(2-aminoethyoxy)ethoxy) phosphazene (PAEP) with different components of multifold charge groups was synthesized by means of introducing imidazole-4-carboxaldehyde (IC) into PAEP through the formation of Schiff base. Though the polymers with primary amino groups (1 degrees) alone or with abundant primary amino groups could bind DNA more efficiently than the ones with mainly or totally secondary (2 degrees) and tertiary (3 degrees) amino groups, all of the polymers could condense DNA into small particles within 100 nm at the N/P ratio of 24. The cell viability of complexes and the pH buffering capacity of polymers increased with substitution degree of IC increasing. Among all the PAEP-based polymers, the highest transfection activity was found for poly(2(2-aminoethyoxy)ethoxy/IC)phosphazene (PAEIC) 18 complexes containing 1 degrees, 2 degrees and 3 degrees amines at a ratio of 3.5:1:1 with 18% substitution degree of IC, which indicated that either the coexistence of 1 degrees,2 degrees and 3 degrees amines or the conjugation of imidazole moiety played an important role in transfection activity. These results suggested that the most efficient gene carrier could be these polymers with 1 degrees,2 degrees and 3 degrees amines at an appropriate ratio, together with the presence of imidazole moiety in a small fraction. (C) 2010 Elsevier B.V. All rights reserved. |
| WOS关键词 | GENE DELIVERY ; IN-VITRO ; DNA ; POLYETHYLENIMINE ; EFFICIENCY ; CHITOSAN ; POLYPLEXES ; VECTORS ; CARRIER ; POLYMER |
| 资助项目 | National Basic Research Program of China[2010CB934000] ; National Basic Research Program of China[2007CB935800] ; National Natural Science Foundation of China[30925041] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program"[2009ZX09501-024] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program"[2009ZX09301-001] ; Shanghai Nanomedicine Program[0852nm05700] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000277795000013 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278900]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Li, Yaping |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Delivery Syst, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yongxin,Zhang, Zhiwen,Chen, Lingli,et al. Effect of multifold charge groups and imidazole-4-carboxaldehyde on physicochemical characteristics and transfection of cationic polyphosphazenes/DNA complexes[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2010,390(2):191-197. |
| APA | Yang, Yongxin,Zhang, Zhiwen,Chen, Lingli,&Li, Yaping.(2010).Effect of multifold charge groups and imidazole-4-carboxaldehyde on physicochemical characteristics and transfection of cationic polyphosphazenes/DNA complexes.INTERNATIONAL JOURNAL OF PHARMACEUTICS,390(2),191-197. |
| MLA | Yang, Yongxin,et al."Effect of multifold charge groups and imidazole-4-carboxaldehyde on physicochemical characteristics and transfection of cationic polyphosphazenes/DNA complexes".INTERNATIONAL JOURNAL OF PHARMACEUTICS 390.2(2010):191-197. |
入库方式： OAI收割
来源：上海药物研究所
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