The characteristics and performance of a multifunctional nanoassembly system for the co-delivery of docetaxel and iSur-pDNA in a mouse hepatocellular carcinoma model
文献类型：期刊论文
| 作者 | Xu, Zhenghong; Zhang, Zhiwen ; Chen, Yi; Chen, Lingli; Lin, Liping; Li, Yaping
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| 刊名 | BIOMATERIALS
 |
| 出版日期 | 2010-02 |
| 卷号 | 31期号:5页码:916-922 |
| 关键词 | Hepatocellular carcinoma Chemotherapy Gene therapy Co-delivery Docetaxel Survivin |
| ISSN号 | 0142-9612 |
| DOI | 10.1016/j.biomaterials.2009.09.103 |
| 文献子类 | Article |
| 英文摘要 | Human hepatocellular carcinoma (HCC) is one of the most causes of cancer-related death and is well known because of resistant to chemotherapeutic drug. Co-delivery of antitumor agent docetaxel and iSur-pDNA, a suppressor of metastatic and resistance-related protein survivin, was postulated to achieve synergistic/combined effect of antitumor drug and gene therapeutics. To valid this hypothesis, a folate-modified multifunctional nanoassembly (FNA) loading both docetaxel and iSur-pDNA was constructed and evaluated as a therapeutic approach for HCC. The FNAs were prepared with folate-modified lipid FA-PEG-DSPE as the target to tumor, protamine sulfate (PS) as the condenser to protect and enhance the nuclear transfer of iSur-pDNA, and DOPE-based lipid envelope as the carrier of doctaxel and PS/DNA complex to achieve their co-delivery and enhance internalization into hepatoma cells. FNAs showed the particle size about 200 nm with encapsulation efficiency >90%. Blank nanoassemblies (BNAs) loading only reporter gene revealed higher transfection efficiency with neglectable cytotoxicity compared with Lipofectamine (TM) 2000, which could result from enhanced cellular uptake via ligand-receptor recognition and efficient nuclear delivery mediated by PS. Cytotoxicity of FNAs against hepatocellular carcinoma cell line BEL 7402 was much higher than either docetaxel or non-docetaxel FNAs (nFNAs) loading only iSur-pDNA, and was also superior to the combined treatment with free docetaxel and nFNAs. Better antitumor efficacy of FNAs with low systemic toxicity was also observed on mouse hepatocellular carcinoma xenograft model. These results suggested that co-delivery of docetaxel and iSur-pDNA with FNAs could be a safer and more efficient strategy for the treatment of locally advanced and metastatic HCC. (C) 2009 Elsevier Ltd. All rights reserved. |
| WOS关键词 | MEDIATED GENE-TRANSFER ; PHASE-II ; IN-VIVO ; CELLS ; SURVIVIN ; THERAPY ; CHEMOTHERAPY ; EXPRESSION ; APOPTOSIS ; CANCER |
| 资助项目 | National Basic Research Program of China[2007CB934000] ; National Basic Research Program of China[2010CB935800] ; National Natural Science Foundation of China[30925041] ; National Natural Science Foundation of China[30901866] ; Important Direction Program of CAS[KJCX2.YW.M02] ; Important Direction Program of CAS[KSCX1-YW-R-21] ; Shanghai Nanomedicine Program[0852nm05700] ; Shanghai Nanomedicine Program[074319117] |
| WOS研究方向 | Engineering ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000273946100017 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278977]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Li, Yaping |
| 作者单位 | Chinese Acad Sci, Ctr Drug Delivery Syst, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Zhenghong,Zhang, Zhiwen,Chen, Yi,et al. The characteristics and performance of a multifunctional nanoassembly system for the co-delivery of docetaxel and iSur-pDNA in a mouse hepatocellular carcinoma model[J]. BIOMATERIALS,2010,31(5):916-922. |
| APA | Xu, Zhenghong,Zhang, Zhiwen,Chen, Yi,Chen, Lingli,Lin, Liping,&Li, Yaping.(2010).The characteristics and performance of a multifunctional nanoassembly system for the co-delivery of docetaxel and iSur-pDNA in a mouse hepatocellular carcinoma model.BIOMATERIALS,31(5),916-922. |
| MLA | Xu, Zhenghong,et al."The characteristics and performance of a multifunctional nanoassembly system for the co-delivery of docetaxel and iSur-pDNA in a mouse hepatocellular carcinoma model".BIOMATERIALS 31.5(2010):916-922. |
入库方式： OAI收割
来源：上海药物研究所
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