中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
The characteristics and performance of a multifunctional nanoassembly system for the co-delivery of docetaxel and iSur-pDNA in a mouse hepatocellular carcinoma model

文献类型:期刊论文

作者Xu, Zhenghong; Zhang, Zhiwen; Chen, Yi; Chen, Lingli; Lin, Liping; Li, Yaping
刊名BIOMATERIALS
出版日期2010-02
卷号31期号:5页码:916-922
关键词Hepatocellular carcinoma Chemotherapy Gene therapy Co-delivery Docetaxel Survivin
ISSN号0142-9612
DOI10.1016/j.biomaterials.2009.09.103
文献子类Article
英文摘要Human hepatocellular carcinoma (HCC) is one of the most causes of cancer-related death and is well known because of resistant to chemotherapeutic drug. Co-delivery of antitumor agent docetaxel and iSur-pDNA, a suppressor of metastatic and resistance-related protein survivin, was postulated to achieve synergistic/combined effect of antitumor drug and gene therapeutics. To valid this hypothesis, a folate-modified multifunctional nanoassembly (FNA) loading both docetaxel and iSur-pDNA was constructed and evaluated as a therapeutic approach for HCC. The FNAs were prepared with folate-modified lipid FA-PEG-DSPE as the target to tumor, protamine sulfate (PS) as the condenser to protect and enhance the nuclear transfer of iSur-pDNA, and DOPE-based lipid envelope as the carrier of doctaxel and PS/DNA complex to achieve their co-delivery and enhance internalization into hepatoma cells. FNAs showed the particle size about 200 nm with encapsulation efficiency >90%. Blank nanoassemblies (BNAs) loading only reporter gene revealed higher transfection efficiency with neglectable cytotoxicity compared with Lipofectamine (TM) 2000, which could result from enhanced cellular uptake via ligand-receptor recognition and efficient nuclear delivery mediated by PS. Cytotoxicity of FNAs against hepatocellular carcinoma cell line BEL 7402 was much higher than either docetaxel or non-docetaxel FNAs (nFNAs) loading only iSur-pDNA, and was also superior to the combined treatment with free docetaxel and nFNAs. Better antitumor efficacy of FNAs with low systemic toxicity was also observed on mouse hepatocellular carcinoma xenograft model. These results suggested that co-delivery of docetaxel and iSur-pDNA with FNAs could be a safer and more efficient strategy for the treatment of locally advanced and metastatic HCC. (C) 2009 Elsevier Ltd. All rights reserved.
WOS关键词MEDIATED GENE-TRANSFER ; PHASE-II ; IN-VIVO ; CELLS ; SURVIVIN ; THERAPY ; CHEMOTHERAPY ; EXPRESSION ; APOPTOSIS ; CANCER
资助项目National Basic Research Program of China[2007CB934000] ; National Basic Research Program of China[2010CB935800] ; National Natural Science Foundation of China[30925041] ; National Natural Science Foundation of China[30901866] ; Important Direction Program of CAS[KJCX2.YW.M02] ; Important Direction Program of CAS[KSCX1-YW-R-21] ; Shanghai Nanomedicine Program[0852nm05700] ; Shanghai Nanomedicine Program[074319117]
WOS研究方向Engineering ; Materials Science
语种英语
WOS记录号WOS:000273946100017
出版者ELSEVIER SCI LTD
源URL[http://119.78.100.183/handle/2S10ELR8/278977]  
专题药物制剂研究中心
通讯作者Li, Yaping
作者单位Chinese Acad Sci, Ctr Drug Delivery Syst, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714
Xu, Zhenghong,Zhang, Zhiwen,Chen, Yi,et al. The characteristics and performance of a multifunctional nanoassembly system for the co-delivery of docetaxel and iSur-pDNA in a mouse hepatocellular carcinoma model[J]. BIOMATERIALS,2010,31(5):916-922.
APA Xu, Zhenghong,Zhang, Zhiwen,Chen, Yi,Chen, Lingli,Lin, Liping,&Li, Yaping.(2010).The characteristics and performance of a multifunctional nanoassembly system for the co-delivery of docetaxel and iSur-pDNA in a mouse hepatocellular carcinoma model.BIOMATERIALS,31(5),916-922.
MLA Xu, Zhenghong,et al."The characteristics and performance of a multifunctional nanoassembly system for the co-delivery of docetaxel and iSur-pDNA in a mouse hepatocellular carcinoma model".BIOMATERIALS 31.5(2010):916-922.

入库方式: OAI收割

来源:上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。