"Intelligent" nanoassembly for gene delivery: In vitro transfection and the possible mechanism
文献类型：期刊论文
| 作者 | Xu, Zhenghong; Chen, Lingli ; Zhang, Zhiwen; Gu, Wangwen; Li, Yaping
|
| 刊名 | INTERNATIONAL JOURNAL OF PHARMACEUTICS
 |
| 出版日期 | 2010-01-04 |
| 卷号 | 383期号:1-2页码:271-276 |
| 关键词 | DNA Gene delivery Nanoassembly Poly(ethylene glycol)-disulfide-DOPE Self-dePEGylation |
| ISSN号 | 0378-5173 |
| DOI | 10.1016/j.ijpharm.2009.09.026 |
| 文献子类 | Article |
| 英文摘要 | A new "intelligent" nanoassembly (INA), consisting of a condensed core of pDNA with protamine sulfate (PS) and a dioleoylphosphatidyl ethanolamine (DOPE)-based lipid envelope containing polyethylene glycol)-disulfide-DOPE (PSD), was designed and investigated. The in vitro release experiment was carried out in solution containing 10 mM of Glutathione, which reflected the redox potential of the intracellular environment. The experimental result indicated that PSD possessed a good ability of self-dePEGylation and could result in efficient release of content in the reductive environment. INAs showed higher transfection efficiency and much lower cytotoxicity compared with Lipofectamine (TM) 2000 on HEK 293 cells. Cellular uptake and subcellular localization, as well as the quantitation of nuclear transfer demonstrated that the superior transfection efficiency of INAs could result from both enhanced cellular uptake mediated by DOPE and efficient nuclear delivery mediated by PS. The biodistribution of INAs in nude mice bearing tumor implied that this PSD-based nanoassembly loading PS/DNA could be a promising gene delivery system for tumor therapy. (C) 2009 Elsevier B.V. All rights reserved. |
| WOS关键词 | LIPOSOMES ; THERAPY ; SYSTEMS ; MICROINJECTION ; NANOPARTICLES ; PROTAMINE |
| 资助项目 | National Basic Research Program of China[2006CB933300] ; National Basic Research Program of China[2007CB935800] ; National Natural Science Foundation of China[30873169] ; National Natural Science Foundation of China[30901866] ; Important Direction Program of CAS[KJCX2.YW.M02] ; Important Direction Program of CAS[KSCX7-YW-R-21] ; Important Direction Program of CAS[KSCX2-YW-R-09] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000272809800036 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278999]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Li, Yaping |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Delivery Syst, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Zhenghong,Chen, Lingli,Zhang, Zhiwen,et al. "Intelligent" nanoassembly for gene delivery: In vitro transfection and the possible mechanism[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2010,383(1-2):271-276. |
| APA | Xu, Zhenghong,Chen, Lingli,Zhang, Zhiwen,Gu, Wangwen,&Li, Yaping.(2010)."Intelligent" nanoassembly for gene delivery: In vitro transfection and the possible mechanism.INTERNATIONAL JOURNAL OF PHARMACEUTICS,383(1-2),271-276. |
| MLA | Xu, Zhenghong,et al.""Intelligent" nanoassembly for gene delivery: In vitro transfection and the possible mechanism".INTERNATIONAL JOURNAL OF PHARMACEUTICS 383.1-2(2010):271-276. |
入库方式： OAI收割
来源：上海药物研究所
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