Chitosan N-betainates/DNA self-assembly nanoparticles for gene delivery: In vitro uptake and transfection efficiency
文献类型：期刊论文
| 作者 | Gao, Yu; Zhang, Zhiwen ; Chen, Lingli; Gu, Wangwen; Li, Yaping
|
| 刊名 | INTERNATIONAL JOURNAL OF PHARMACEUTICS
 |
| 出版日期 | 2009-04-17 |
| 卷号 | 371期号:1-2页码:156-162 |
| 关键词 | Chitosan N-betainates Gene vector DNA Chitosan Nanoparticles |
| ISSN号 | 0378-5173 |
| DOI | 10.1016/j.ijpharm.2008.12.012 |
| 文献子类 | Article |
| 英文摘要 | The aim of this work is to investigate the effect of betaine substitution degree of chitosan N-betainates (CsB) on cellular uptake, cytotoxicity and transfection efficiency of CsB/DNA complex nanoparticles (CsBNs) against COS-7 and MDA-MB-468 cells. The polymers with three substitution degrees (CsB12, CsB47 and CsB85) complexed with pDNA formed CsBN12s, CsBN47s and CsBN85s. The CsBNs showed less pH dependency with smaller particle size and higher zeta potential than that of chitosan/pDNA complex nanoparticles (CsNs) at neutral pH. CsBN85s showed stronger cellular uptake than that of CsBN47s or CsBN12s. CsBNs showed higher cytotoxicity than CsNs, and a trend increasing toxicity with substitution degree increasing. In COS-7 cells, the transfection efficiency increased with the substitution degree increasing, while the opposite result was observed in MDA-MB-468 cells. Chitosan modified with betaine could increase its ability to facilitate DNA uptake and its cytotoxicity, both of which showed the influence on transfection efficiency. It was able to increase cellular uptake and transfection efficiency of complex nanoparticles in COS-7 cells to increase betaine substitution of CsB, however, the higher sensitivity of MDA-MB-468 cells to CsBs led to decreased transfection efficiency due to the increased cytotoxicity with betaine substitution increasing. The predominant role of cellular uptake or toxicity in affecting transfection efficiency was different in two cell lines. These results provided an important guidepost for further development of chitosan derivatives/pDNA complexes as non-viral gene vectors. (C) 2009 Published by Elsevier B.V. |
| WOS关键词 | TRIMETHYL CHITOSAN ; BLOCK-COPOLYMERS ; CHLORIDE ; CYTOTOXICITY ; VECTORS ; VIABILITY ; THERAPY ; CELLS ; MICE |
| 资助项目 | National Basic Research Program of China[2009CB930300] ; National Basic Research Program of China[2007CB935804] ; National Natural Science Foundation of China[30873169] ; Important Direction Program of CAS[KJCX2.YW.M02] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000265361300021 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279263]  |
| 专题 | 药物制剂研究中心 上海中药现代化研究中心 |
| 通讯作者 | Li, Yaping |
| 作者单位 | Chinese Acad Sci, Ctr Drug Delivery Syst, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Yu,Zhang, Zhiwen,Chen, Lingli,et al. Chitosan N-betainates/DNA self-assembly nanoparticles for gene delivery: In vitro uptake and transfection efficiency[J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS,2009,371(1-2):156-162. |
| APA | Gao, Yu,Zhang, Zhiwen,Chen, Lingli,Gu, Wangwen,&Li, Yaping.(2009).Chitosan N-betainates/DNA self-assembly nanoparticles for gene delivery: In vitro uptake and transfection efficiency.INTERNATIONAL JOURNAL OF PHARMACEUTICS,371(1-2),156-162. |
| MLA | Gao, Yu,et al."Chitosan N-betainates/DNA self-assembly nanoparticles for gene delivery: In vitro uptake and transfection efficiency".INTERNATIONAL JOURNAL OF PHARMACEUTICS 371.1-2(2009):156-162. |
入库方式： OAI收割
来源：上海药物研究所
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