Lipid-Based Liquid Crystalline Nanoparticles Facilitate Cytosolic Delivery of siRNA via Structural Transformation
文献类型：期刊论文
| 作者 | He, Shufang1,3; Fan, Weiwei1,3; Wu, Na3; Zhu, Jingjing3; Miao, Yunqiu3; Miao, Xiaran2; Li, Feifei3; Zhang, Xinxin3 ; Gan, Yong3
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| 刊名 | NANO LETTERS
 |
| 出版日期 | 2018-04 |
| 卷号 | 18期号:4页码:2411-2419 |
| 关键词 | Lipids liquid crystalline nanoparticles siRNA delivery endosomal escape synthetic lethality |
| ISSN号 | 1530-6984 |
| DOI | 10.1021/acs.nanolett.7b05430 |
| 文献子类 | Article |
| 英文摘要 | RNA interference (RNAi) technology has shown great promise for the treatment of cancer and other genetic disorders. Despite the efforts to increase the target tissue distribution, the safe and effective delivery of siRNA to the diseased cells with sufficient cytosolic transport is another critical factor for successful RNAi clinical application. Here, the constructed lipid-based liquid crystalline nanoparticles, called nano-Transformers, can transform the structure in the intracellular acidic environment and perform high-efficient siRNA delivery for cancer treatment. The developed nano-Transformers have satisfactory siRNA loading efficiency and low cytotoxicity. Different from the traditional cationic nanocarriers, the endosomal membrane fusion induced by the conformational transition of lipids contributes to the easy dissociation of siRNA from nanocarriers and direct release of free siRNA into cytoplasm. We show that transfection with cyclin-dependent kinase 1 (CDK1)-siRNA-loaded nano-Transformers causes up to 95% reduction of relevant mRNA in vitro and greatly inhibits the tumor growth without causing any immunogenic response in vivo. This work highlights that the lipid-based nano-Transformers may become the next generation of siRNA delivery system with higher efficacy and improved safety profiles. |
| WOS关键词 | GENE DELIVERY ; DRUG-DELIVERY ; VIRAL VECTORS ; NONVIRAL VECTORS ; THERAPY ; THERAPEUTICS ; NANOCARRIERS ; RNAI ; PH ; INHIBITION |
| 资助项目 | National Natural Science Foundation of China[81571796] ; National Natural Science Foundation of China[61433017] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[2015229] ; SA-SIBS Scholarship Program[00000000] |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000430155900031 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279828]  |
| 专题 | 药物制剂研究中心 |
| 通讯作者 | Zhang, Xinxin; Gan, Yong |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Appl Phys, Shanghai Synchrotron Radiat Facil, Shanghai 201204, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | He, Shufang,Fan, Weiwei,Wu, Na,et al. Lipid-Based Liquid Crystalline Nanoparticles Facilitate Cytosolic Delivery of siRNA via Structural Transformation[J]. NANO LETTERS,2018,18(4):2411-2419. |
| APA | He, Shufang.,Fan, Weiwei.,Wu, Na.,Zhu, Jingjing.,Miao, Yunqiu.,...&Gan, Yong.(2018).Lipid-Based Liquid Crystalline Nanoparticles Facilitate Cytosolic Delivery of siRNA via Structural Transformation.NANO LETTERS,18(4),2411-2419. |
| MLA | He, Shufang,et al."Lipid-Based Liquid Crystalline Nanoparticles Facilitate Cytosolic Delivery of siRNA via Structural Transformation".NANO LETTERS 18.4(2018):2411-2419. |
入库方式： OAI收割
来源：上海药物研究所
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