Characterization of two homogalacturonan pectins with immunomodulatory activity from green tea
文献类型：期刊论文
| 作者 | Wang, Huijun3; Wei, Guodong4; Liu, Fei3; Banerjee, Gautam5; Joshi, Manoj1; Bligh, S W Annie2; Shi, Songshan3; Lian, Hui3; Fan, Hongwei3; Gu, Xuelan4 |
| 刊名 | International journal of molecular sciences
 |
| 出版日期 | 2014-06-04 |
| 卷号 | 15期号:6页码:9963-78 |
| ISSN号 | 1422-0067 |
| DOI | 10.3390/ijms15069963 |
| 文献子类 | Article |
| 英文摘要 | Two natural homogalacturonan (HG) pectins (MW ca. 20 kDa) were isolated from green tea based on their immunomodulatory activity. The crude tea polysaccharides (TPS1 and TPS2) were obtained from green tea leaves by hot water extraction and followed by 40% and 70% ethanol precipitation, respectively. Two homogenous water soluble polysaccharides (TPS1-2a and TPS1-2b) were obtained from TPS1 after purification with gel permeation, which gave a higher phagocytic effect than TPS2. A combination of composition, methylation and configuration analyses, as well as NMR (nuclear magnetic resonance) spectroscopy revealed that TPS1-2a and TPS1-2b were homogalacturonan (HG) pectins consisting of a backbone of 1,4-linked α-D-galacturonic acid (GalA) residues with 28.4% and 26.1% of carboxyl groups as methyl ester, respectively. The immunological assay results demonstrated that TPS1-2, which consisted mainly of HG pectins, showed phagocytosis-enhancing activity in HL-60 cells. |
| 语种 | 英语 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/266626]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Wang, Huijun; Wei, Guodong; Liu, Fei; Banerjee, Gautam; Joshi, Manoj; Bligh, S W Annie; Shi, Songshan; Lian, Hui; Fan, Hongwei; Gu, Xuelan; Wang, Shunchun |
| 作者单位 | 1.Unilever R&D Bangalore, 66 Main Road, Whitefield, Bangalore 560066, India. Joshi; 2.Department of Complementary Medicine, Faculty of Science and Technology, University of Westminster, London W1W 6UW, UK. a 3.The MOE Key Laboratory for Standardization of Chinese Medicines and the SATCM Key Laboratory for New Resources and Quality Evaluation of Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China; 4.Unilever R&D Shanghai, 66 Lin Xin Road, Linkong Economic Development Zone, Shanghai 200335, China; 5.Unilever R&D Bangalore, 66 Main Road, Whitefield, Bangalore 560066, India. Gautam; |
| 推荐引用方式 GB/T 7714 | Wang, Huijun,Wei, Guodong,Liu, Fei,et al. Characterization of two homogalacturonan pectins with immunomodulatory activity from green tea[J]. International journal of molecular sciences,2014,15(6):9963-78. |
| APA | Wang, Huijun.,Wei, Guodong.,Liu, Fei.,Banerjee, Gautam.,Joshi, Manoj.,...&Wang, Shunchun.(2014).Characterization of two homogalacturonan pectins with immunomodulatory activity from green tea.International journal of molecular sciences,15(6),9963-78. |
| MLA | Wang, Huijun,et al."Characterization of two homogalacturonan pectins with immunomodulatory activity from green tea".International journal of molecular sciences 15.6(2014):9963-78. |
入库方式： OAI收割
来源：上海药物研究所
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