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Determination of the binding rate of rat plasma protein with salvianolic acid B
文献类型：期刊论文
| 作者 | JING Chun-jie2; CHEN Xiao-hui2; LIU Xuan1; BI Kai-shun2; GUO De-an1
|
| 刊名 | Acta Pharmaceutica Sinica
 |
| 出版日期 | 2010 |
| 卷号 | 45期号:3页码:343-346 |
| 关键词 | salvianolic acid B:protein binding rate equilibrium dialysis HPLC |
| ISSN号 | 0513-4870 |
| 其他题名 | 丹酚酸B与大鼠血浆蛋白结合率的测定 |
| 文献子类 | Article |
| 英文摘要 | This paper iS aimed to report the development of a method for the determination of the binding rate of plasma protein with salvianolie acid B.In vitro.equilibrium dialysis method was used to imitate the binding process between salvianolic acid B and plasma protein,in vivo,ultrafiltration method Was used and the binding rate with HPLC was determined.Plasma samples were treated with methanol to precipitate the protein,and the buffer solution was directly determined after filtering.The calibration curve of the buffer solution was liner in the rang of 0.5-20mug·mL~(-1).The calibration curve of the plasma was linear in the rang of 2-200mug·mL~(-1).The extract reovvery was 68.6%-81.9%.RSDs of intra-and inter-day precisions wrer all less than 8.5%.The binding rates of plasma protein with salvianolic acid B in vitro was 75.2%and in vivo was 92.1%.This paper shows the high binding power of salvianolic acid B to plasma protein with high sensitivity,good reproduction,simple management and fulfilling the requirement. |
| 资助项目 | 国家科技重大专项课题资助项目[00000000] ; "重大新药创制"科技重大专项资助项目[00000000] |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:3824704 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267964]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | GUO De-an |
| 作者单位 | 1.Shanghai Research Center for Modernization of Traditional Chinese Medicine,Shanghai Institute of Materia Medica,Chinese Academy of Sciences, Shanghai 201203, China. 2.School of Pharmacy,Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China.; |
| 推荐引用方式 GB/T 7714 | JING Chun-jie,CHEN Xiao-hui,LIU Xuan,et al. Determination of the binding rate of rat plasma protein with salvianolic acid B[J]. Acta Pharmaceutica Sinica,2010,45(3):343-346. |
| APA | JING Chun-jie,CHEN Xiao-hui,LIU Xuan,BI Kai-shun,&GUO De-an.(2010).Determination of the binding rate of rat plasma protein with salvianolic acid B.Acta Pharmaceutica Sinica,45(3),343-346. |
| MLA | JING Chun-jie,et al."Determination of the binding rate of rat plasma protein with salvianolic acid B".Acta Pharmaceutica Sinica 45.3(2010):343-346. |
入库方式： OAI收割
来源：上海药物研究所
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