A UPLC/MS/MS method for determination of protosappanin B in rat plasma and its application of a pharmacokinetic and bioavailability study
文献类型：期刊论文
| 作者 | Chen, Wei-Ying3; Zhou, Xian-Zhen2; Wu, Li-Lan3; Wu, Yun-Shan3; Wang, Shu-Mei2; Liu, Bo3; Guo, De-An1,3
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| 刊名 | BIOMEDICAL CHROMATOGRAPHY
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| 出版日期 | 2017-07 |
| 卷号 | 31期号:7 |
| ISSN号 | 0269-3879 |
| 关键词 | Bioavailability Pharmacokinetic protosappanin B UPLC-MS/MS |
| DOI | 10.1002/bmc.3919 |
| 文献子类 | Article |
| 英文摘要 | Caesalpinia sappan L. is a traditional medicinal plant which is used for promoting blood circulation and cerebral apoplexy therapy in China. Previous reports showed that the extracts of Caesalpinia sappan L. could exert vasorelaxant activity and anti-inflammation activity. Protosappanin B is a major constituent of C. sappan L., and showed several important bioactivities. The separation was achieved by an Acquity UPLC BEH Symmetry Shield RP18 column (1.7 mu m, 2.1 x 100 mm) column with the gradient mobile phase consisting of 5mM ammonium acetate aqueous solution and acetonitrile. Detection was carried out by using negative-ion electrospray tandem mass spectrometry via multiple reaction monitoring. Plasma samples were preprocessed by an extraction with ethyl acetate, and apigenin was used as internal standard. The current UPLC-MS/MS assay was validated for linearity, accuracy, intraday and interday precisions, stability, matrix effects and extraction recovery. After oral and intravenous administration, the main pharmacokinetic parameters were as follows: peak concentrations, 83.5 +/- 46.2 and 1329.6 +/- 343.6 ng/mL; areas under the concentration-time curve, 161.9 +/- 69.7 and 264.9 +/- 56.3 mu g h/L; and half-lives, 3.4 +/- 0.9 and 0.3 +/- 0.1 h, respectively. The absolute bioavailability in rats of protosappanin B was 12.2%. The method has been successfully applied to a pharmacokinetic and bioavailability study of protosappanin B in rats. |
| WOS关键词 | CAESALPINIA-SAPPAN ; BRAZILIN ; LIGNUM ; HEARTWOOD |
| 资助项目 | Guangdong Provincial Science and Technology Project[2015A020211025] ; Guangdong Provincial Science and Technology Project[2014A020221035] ; National Natural Science Foundation of China[81473413] ; National Natural Science Foundation of China[81274060] ; National Natural Science Foundation of China[81202398] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000404968600014 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272588]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Liu, Bo; Guo, De-An |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai, Peoples R China 2.Guangdong Pharmaceut Univ, SATCMState Adm TCM, Key Unit Chinese Med Digitalizat Qual Evaluat, Guangzhou, Guangdong, Peoples R China; 3.Guangzhou Univ Chinese Med, Guangdong Prov Hosp Chinese Med, Clin Coll 2, Guangzhou, Guangdong, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Chen, Wei-Ying,Zhou, Xian-Zhen,Wu, Li-Lan,et al. A UPLC/MS/MS method for determination of protosappanin B in rat plasma and its application of a pharmacokinetic and bioavailability study[J]. BIOMEDICAL CHROMATOGRAPHY,2017,31(7). |
| APA | Chen, Wei-Ying.,Zhou, Xian-Zhen.,Wu, Li-Lan.,Wu, Yun-Shan.,Wang, Shu-Mei.,...&Guo, De-An.(2017).A UPLC/MS/MS method for determination of protosappanin B in rat plasma and its application of a pharmacokinetic and bioavailability study.BIOMEDICAL CHROMATOGRAPHY,31(7). |
| MLA | Chen, Wei-Ying,et al."A UPLC/MS/MS method for determination of protosappanin B in rat plasma and its application of a pharmacokinetic and bioavailability study".BIOMEDICAL CHROMATOGRAPHY 31.7(2017). |
入库方式： OAI收割
来源：上海药物研究所
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