Proteomic analysis of differential protein expression in rat platelets treated with notoginsengnosides
文献类型：期刊论文
| 作者 | Yao, Yan1,2; Wu, Wan-Ying2 ; Guan, Shu-Hong2; Jiang, Bao-Hong2; Yang, Min2; Chen, Xiao-Hui1; Bi, Kai-Shun1; Liu, Xuan2; Guo, De-An2
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| 刊名 | PHYTOMEDICINE
 |
| 出版日期 | 2008-10 |
| 卷号 | 15期号:10页码:800-807 |
| 关键词 | Panax notoginseng Sanqi Notoginsengnosides Platelet aggregation Proteomics |
| ISSN号 | 0944-7113 |
| DOI | 10.1016/j.phymed.2008.06.013 |
| 文献子类 | Article |
| 英文摘要 | Sanqi, the root of Panax notoginseng, is a popularly used traditional Chinese medicine with cardiovascular effects. Notoginsengnosides (NG) isolated from Sanqi could inhibit ADP-induced platelet aggregation of rat washed platelets. To identify the possible target proteins of NG in platelets, two-dimensional gel clectrophoresis (2-DE)-based comparative proteomics was performed and proteins altered in expressional level after NG treatment were identified by MALDI-TOF MS/MS. Treatment of 200 mu g/ml NG caused regulation of the levels of 12 proteins, which play important roles in platelet activation, oxidative stress and cytoskeleton. In the NG-treated platelets, there were increase in the levels of growth factor receptor-bound protein 2 (Grb2), thrombospondin 1, tubulin alpha 6 and decrease in the levels of thioredoxin, Cu-Zn superoxide dismutase, DJ-1 protein, peroxiredoxin 3, thioredoxin-like protein 2, ribonuclease inhibitor, potassium channel subfamily V member 2, myosin regulatory light chain 9 and laminin receptor 1. The change in the levels of these proteins caused by NG treatment might contribute to the inhibitive effect of NG on platelet aggregation. Furthermore, analysis of the reactive oxygen species (ROS) level indicated that NG could decrease the ROS level in platelets. The regulation of ROS level might play important role in the effect of NG on platelets. (C) 2008 Elsevier GmbH. All rights reserved. |
| WOS关键词 | PANAX-NOTOGINSENG ; INHIBITION ; AGGREGATION ; GINSENG ; DOMAIN ; THROMBIN ; SAPONINS ; BLOOD ; CELLS |
| 资助项目 | Chinese Academy of Sciences[SIMM0709QN-14] ; Ministry of Science and Technology of the People's Republic of China[2006DFB31860] ; Science and Technology Commission of Shanghai Municipality[064319052] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000260352200003 |
| 出版者 | ELSEVIER GMBH, URBAN & FISCHER VERLAG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272801]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Liu, Xuan |
| 作者单位 | 1.Shenyang Pharmaceut Univ, Shenyang 110016, Liaoning, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Yao, Yan,Wu, Wan-Ying,Guan, Shu-Hong,et al. Proteomic analysis of differential protein expression in rat platelets treated with notoginsengnosides[J]. PHYTOMEDICINE,2008,15(10):800-807. |
| APA | Yao, Yan.,Wu, Wan-Ying.,Guan, Shu-Hong.,Jiang, Bao-Hong.,Yang, Min.,...&Guo, De-An.(2008).Proteomic analysis of differential protein expression in rat platelets treated with notoginsengnosides.PHYTOMEDICINE,15(10),800-807. |
| MLA | Yao, Yan,et al."Proteomic analysis of differential protein expression in rat platelets treated with notoginsengnosides".PHYTOMEDICINE 15.10(2008):800-807. |
入库方式： OAI收割
来源：上海药物研究所
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