Identification of major alkaloids and steroidal saponins in rat serum by HPLC-diode array detection-MS/MS following oral administration of Huangbai-Zhimu herb-pair Extract
文献类型：期刊论文
| 作者 | Ma, Chunhui; Fan, Mingsong; Tang, Yihong; Li, Zhixiong ; Sun, Zhaolin; Ye, Guan; Huang, Chenggang
|
| 刊名 | BIOMEDICAL CHROMATOGRAPHY
 |
| 出版日期 | 2008-08 |
| 卷号 | 22期号:8页码:835-850 |
| 关键词 | Phellodendron chinensis Anemarrhena asphodeloides alkaloid steroidal saponins serum |
| ISSN号 | 0269-3879 |
| DOI | 10.1002/bmc.1000 |
| 文献子类 | Article |
| 英文摘要 | Huangbai-Zhimu herb-pair (HBZMHP) is a widely used Chinese traditional medicine formula in treating various diseases; however, its active components have remained unknown. In this paper, serum chemistry and combined high-performance liquid chromatography (HPLC), diode-array detection and mass-spectrometry (MS) techniques were used to study the constituents of HBZMHP extract absorbed into rat serum after oral administration. A total of nine characteristic HPLC peaks in the TIC chromatograms were identified as magnoflorine (1), menisperine (2), palmatine (3), berberine (4), timosaponin N or timosaponin El (5), timosaponin D (6), timosaponin 13111, anemarsaponin C or xilingsaponin B (7) timosaponin BIII (8) and timosaponin AIII (9). All of the identified peaks were constituents of HBZMHP extract. The results narrow the range of active compounds to be found in HBZMHP extract, and pave the way for the follow-up action mechanism research. Copyright (C) 2008 John Wiley & Sons, Ltd. |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; TRADITIONAL CHINESE MEDICINE ; NECROSIS-FACTOR-ALPHA ; LC-MS/MS ; SUPEROXIDE GENERATION ; ANEMARRHENAE RHIZOMA ; PLATELET-AGGREGATION ; HUMAN NEUTROPHILS ; HUMAN BLOOD ; METABOLITES |
| 资助项目 | National Natural Science Foundation of China[30701037] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000258541700004 |
| 出版者 | JOHN WILEY & SONS LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272846]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Huang, Chenggang |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Chunhui,Fan, Mingsong,Tang, Yihong,et al. Identification of major alkaloids and steroidal saponins in rat serum by HPLC-diode array detection-MS/MS following oral administration of Huangbai-Zhimu herb-pair Extract[J]. BIOMEDICAL CHROMATOGRAPHY,2008,22(8):835-850. |
| APA | Ma, Chunhui.,Fan, Mingsong.,Tang, Yihong.,Li, Zhixiong.,Sun, Zhaolin.,...&Huang, Chenggang.(2008).Identification of major alkaloids and steroidal saponins in rat serum by HPLC-diode array detection-MS/MS following oral administration of Huangbai-Zhimu herb-pair Extract.BIOMEDICAL CHROMATOGRAPHY,22(8),835-850. |
| MLA | Ma, Chunhui,et al."Identification of major alkaloids and steroidal saponins in rat serum by HPLC-diode array detection-MS/MS following oral administration of Huangbai-Zhimu herb-pair Extract".BIOMEDICAL CHROMATOGRAPHY 22.8(2008):835-850. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。