Comparative analysis on microbial and rat metabolism of ginsenoside Rb-1 by high-performance liquid chromatography coupled with tandem mass spectrometry
文献类型：期刊论文
| 作者 | Chen, Guangtong1,2; Yang, Min2; Song, Yan1; Lu, Zhiqiang2; Zhang, Jinqiang2; Huang, Huilian2; Guan, Shuhong2 ; Wu, Lijun1; Guo, De-an2
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| 刊名 | BIOMEDICAL CHROMATOGRAPHY
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| 出版日期 | 2008-07 |
| 卷号 | 22期号:7页码:779-785 |
| 关键词 | ginsenoside Rb-1 biotransformation metabolism HPLC-MS/MS |
| ISSN号 | 0269-3879 |
| DOI | 10.1002/bmc.1001 |
| 文献子类 | Article |
| 英文摘要 | Ginsenoside Rb-1 is an active protopanaxadiol saponin from Partax species. In order to compare the similarities and differences of microbial and mammalian metabolisms of ginsenoside Rb-1 the microbial transformation by Acremonium strictum and metabolism in rats were comparatively studied. Microbial transformation of ginsenoside Rb-1 by Acremonium strictum AS 3.2058 resulted in the formation of eight metabolites. Ten metabolites (M1-M10) were detected from the in vivo study in rats and eight of them were identified as the same compounds as those obtained from microbial metabolism by liquid chromatography-tandem mass spectrometry analysis and comparison with reference standards obtained from microbial metabolism. Their structures were identified as ginsenoside Rd, gypenoside XVII, 20(S)-ginsenoside Rg(3), 20(R)-ginsenoside Rg(3), ginsenoside F-2, compound K, 12 beta-hydroxydammar-3-one-20(S)-O-beta-D-glucopyranoside, and 25-hydroxyl-(E)-20(22)-ene-ginsenoside Rg(3), respectively. The structures of the additional two metabolites were tentatively characterized as 20(22),24-diene-ginsenoside Rg(3), and 25-hydroxyginsenoside Rd by HPLC-MS/MS analysis. M7-M10 are the first four reported metabolites in vivo. The time course of rat metabolism of ginsenoside Rb-1 was also investigated. Copyright (c) 2008 John Wiley & Sons, Ltd. |
| WOS关键词 | PANAX-QUINQUEFOLIUS-L. ; PENICILLIUM-JANTHINELLUM ; ASPERGILLUS-NIGER ; BUFADIENOLIDES ; IDENTIFICATION ; TRANSFORMATION ; EXTRACTS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000257901300014 |
| 出版者 | JOHN WILEY & SONS LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272870]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Guo, De-an |
| 作者单位 | 1.Shenyang Pharmaceut Univ, Coll Tradit Chinese Mat Med, Shenyang 110016, Liaoning, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Chen, Guangtong,Yang, Min,Song, Yan,et al. Comparative analysis on microbial and rat metabolism of ginsenoside Rb-1 by high-performance liquid chromatography coupled with tandem mass spectrometry[J]. BIOMEDICAL CHROMATOGRAPHY,2008,22(7):779-785. |
| APA | Chen, Guangtong.,Yang, Min.,Song, Yan.,Lu, Zhiqiang.,Zhang, Jinqiang.,...&Guo, De-an.(2008).Comparative analysis on microbial and rat metabolism of ginsenoside Rb-1 by high-performance liquid chromatography coupled with tandem mass spectrometry.BIOMEDICAL CHROMATOGRAPHY,22(7),779-785. |
| MLA | Chen, Guangtong,et al."Comparative analysis on microbial and rat metabolism of ginsenoside Rb-1 by high-performance liquid chromatography coupled with tandem mass spectrometry".BIOMEDICAL CHROMATOGRAPHY 22.7(2008):779-785. |
入库方式： OAI收割
来源：上海药物研究所
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