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High performance liquid chromatographic method for the determination and pharmacokinetic studies of oxyresveratrol and resveratrol in rat plasma after oral administration of Smilax china extract

文献类型：期刊论文

作者Huang, Huilian1,2; Zhang, Jinqiang2; Chen, Guangtong2; Lu, Zhiqiang2; Wang, Xiaoming2; Sha, Na2; Shao, Bo2; Li, Ping1; Guo, De-an2
刊名BIOMEDICAL CHROMATOGRAPHY
出版日期2008-04
卷号22期号:4页码:421-427
ISSN号0269-3879
关键词Smilax china oxyresveratrol resveratrol HPLC pharmacokinetics
DOI10.1002/bmc.950
文献子类Article
英文摘要A sensitive and simple HPLC method has been developed and validated for the determination of oxyresveratrol (trans-2,4,3',5'-tetrahydroxystilbene, OXY) and resveratrol (trans-3,5,4'-trihydroxystilbene, RES) in rat plasma. The plasma samples were extracted with ethyl acetate and analyzed using HPLC on an Aglient Zorbax SB-C-18 column (250 x 4.6 mm, 5 mu m) at a wavelength 320 nm, with a linear gradient of (A) acetonitrile and (B) 0.5% aqueous acetic acid (v/v), at a flow rate of 1.0 mL/min. The method was linear over the range of 0.1265-25.3 mu g/mL for OXY and 0.117-23.4 mu g/mL for RES. The extraction recovery for OXY, RES and internal standard ranged from 71.1 to 88.3%. The intra- and inter-day precisions were better than 10%, and the accuracy ranged from 89 to 108%. The validated method was used to study the pharmacokinetic profiles of OXY and RES in rat plasma after oral administration of Smilax china root extract. Copyright (c) 2007 John Wiley & Sons, Ltd.
WOS关键词ISCHEMIA-REPERFUSION INJURY ; DALBERGIA-ODORIFERA EXTRACT ; TANDEM MASS-SPECTROMETRY ; RED WINE ; ANTIPLATELET ACTIVITY ; HPLC DETERMINATION ; TRANS-RESVERATROL ; MAJOR FLAVONOIDS ; LDL OXIDATION ; MORUS-ALBA
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
语种英语
出版者JOHN WILEY & SONS LTD
WOS记录号WOS:000255133300013
源URL[http://119.78.100.183/handle/2S10ELR8/272946]  
专题上海中药现代化研究中心
通讯作者Guo, De-an
作者单位1.China Pharmaceut Univ, Coll Tradit Chinese Mat Med, Nanjing 210009, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
推荐引用方式
GB/T 7714		 Huang, Huilian,Zhang, Jinqiang,Chen, Guangtong,et al. High performance liquid chromatographic method for the determination and pharmacokinetic studies of oxyresveratrol and resveratrol in rat plasma after oral administration of Smilax china extract[J]. BIOMEDICAL CHROMATOGRAPHY,2008,22(4):421-427.
APA Huang, Huilian.,Zhang, Jinqiang.,Chen, Guangtong.,Lu, Zhiqiang.,Wang, Xiaoming.,...&Guo, De-an.(2008).High performance liquid chromatographic method for the determination and pharmacokinetic studies of oxyresveratrol and resveratrol in rat plasma after oral administration of Smilax china extract.BIOMEDICAL CHROMATOGRAPHY,22(4),421-427.
MLA Huang, Huilian,et al."High performance liquid chromatographic method for the determination and pharmacokinetic studies of oxyresveratrol and resveratrol in rat plasma after oral administration of Smilax china extract".BIOMEDICAL CHROMATOGRAPHY 22.4(2008):421-427.

入库方式： OAI收割

来源：上海药物研究所

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