Differential protein expression in mouse splenic mononuclear cells treated with polysaccharides from spores of Ganoderma lucidum
文献类型：期刊论文
| 作者 | Ma, Chao; Guan, Shu-Hong ; Yang, Min; Liu, Xuan; Guo, De-An
|
| 刊名 | PHYTOMEDICINE
 |
| 出版日期 | 2008-04 |
| 卷号 | 15期号:4页码:268-276 |
| 关键词 | Ganoderma lucidum polysaccharides proteomics mononuclear cell immuno-modulation |
| ISSN号 | 0944-7113 |
| DOI | 10.1016/j.phymed.2007.11.015 |
| 文献子类 | Article |
| 英文摘要 | Polysaccharides were one of the main components of Ganoderma lucidum, a medicinal mushroom well known for its immuno-modulation effects. In the present study, we demonstrated that polysaccharides extracted from Ganoderma lucidum spores (GL-SP) could stimulate splenic mononuclear cells (MNCs) proliferation and cytokine production. To identify the possible cellular targets of GL-SP in MNCs, two-dimensional gel electrophoresis (2-DE)-based comparative proteomics was performed and proteins altered in expressional level after GL-SP treatment were identified by MALDI-TOF MS/MS. Ten proteins with > 2-fold increase or decrease expression in GL-SP-treated MNCs compared with control were found and further identified by MALDI-TOF MS/MS analysis and database searching. In the GL-SP-treated MNCs, there were increases in the expression of myosin regulatory light chain 2-A, Rho GDP dissociation inhibitor beta, T-cell-specific GTPase, phosphatidylinositol transfer protein a, and decreases in the expression of apoptosis-associated speck-like protein containing a CARD, 14-3-3 tau, beta-actin, tubulin alpha 2, copine 1, and gamma-actin. The results of the present study help to provide insight into the immuno-modulating activities of GL-SP. (C) 2007 Elsevier GmbH. All rights reserved. |
| WOS关键词 | MACROPHAGES ; ACTIVATION ; ALPHA |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000255889800006 |
| 出版者 | ELSEVIER GMBH, URBAN & FISCHER VERLAG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272952]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Liu, Xuan |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Chao,Guan, Shu-Hong,Yang, Min,et al. Differential protein expression in mouse splenic mononuclear cells treated with polysaccharides from spores of Ganoderma lucidum[J]. PHYTOMEDICINE,2008,15(4):268-276. |
| APA | Ma, Chao,Guan, Shu-Hong,Yang, Min,Liu, Xuan,&Guo, De-An.(2008).Differential protein expression in mouse splenic mononuclear cells treated with polysaccharides from spores of Ganoderma lucidum.PHYTOMEDICINE,15(4),268-276. |
| MLA | Ma, Chao,et al."Differential protein expression in mouse splenic mononuclear cells treated with polysaccharides from spores of Ganoderma lucidum".PHYTOMEDICINE 15.4(2008):268-276. |
入库方式： OAI收割
来源：上海药物研究所
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