中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 上海中药现代化研究中心
Simultaneous determination and pharmacokinetics of five bufadienolides in rat plasma after oral administration of Chansu extract by SPE-HPLC method

文献类型：期刊论文

作者Liang, Yan1,2; Liu, Ai-hua1; Qin, Song1; Sun, Hang-hao1; Yang, Min1; Li, Ping2; Guo, De-an1,2
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
出版日期2008-02-13
卷号46期号:3页码:442-448
关键词Venenum Bufonis bufadienolides SPE-HPLC pharmacokinetics rat plasma
ISSN号0731-7085
DOI10.1016/j.jpba.2007.11.001
文献子类Article
英文摘要A sensitive and rapid solid-phase extraction-high performance liquid chromatography (SPE-HPLC) method has been developed for the determination of five bufadienolides, arenobufagin, teliocinobufagin, cinobufotalin, cinobufagin and resibufogenin in rat plasma and applied to a pharmacokinetic study in rats after oral administration of Chansu extract (Venenum Bufonis). Plasma samples were pretreated with solid-phase extraction using Extract-Clean (TM) cartridges, and the extracts were analyzed by a reversed-phase C-18 column on a HPLC system with photodiode array detection (DAD). The calibration curves were linear over the range of 0.10-1.66 mu g/ml for arenobufagin, 0.03-1.20 mu g/ml for telocinobufagin, 0.01-0.62 mu g/ml for cinobufotalin, 0.03-0.70 mu g/ml for cinobufagin and 0.02-2.57 mu g/ml for resibufogenin, respectively. The limit of quantification was 1.1 ng/ml for arenobufagin, 0.3 ng/ml for telocinobufagin, 9.7 ng/ml for cinobufotalin, 8.8 ng/ml for cinobufagin, 7.7 ng/ml for resibufogenin, respectively. The established method could be easily applied to the determination and pharmacokinetic studies of five bufadienolides in rat plasma after oral administration of Chansu extract. (c) 2007 Elsevier B.V. All rights reserved.
WOS关键词PERFORMANCE LIQUID-CHROMATOGRAPHY ; CYTOTOXIC BUFADIENOLIDES ; MICROBIAL HYDROXYLATION ; PSEUDOMONAS-AERUGINOSA ; CATHARANTHUS-ROSEUS ; MASS-SPECTROMETRY ; CHINESE MEDICINE ; TOAD VENOM ; CINOBUFAGIN ; RESIBUFOGENIN
WOS研究方向Chemistry ; Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000253100400004
出版者PERGAMON-ELSEVIER SCIENCE LTD
源URL[http://119.78.100.183/handle/2S10ELR8/272980]  
专题上海中药现代化研究中心
通讯作者Guo, De-an
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China
2.China Pharmaceut Univ, Dept Pharmacognosy & Pharmaceut Bot, Nanjing 210009, Peoples R China;
推荐引用方式
GB/T 7714		 Liang, Yan,Liu, Ai-hua,Qin, Song,et al. Simultaneous determination and pharmacokinetics of five bufadienolides in rat plasma after oral administration of Chansu extract by SPE-HPLC method[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2008,46(3):442-448.
APA Liang, Yan.,Liu, Ai-hua.,Qin, Song.,Sun, Hang-hao.,Yang, Min.,...&Guo, De-an.(2008).Simultaneous determination and pharmacokinetics of five bufadienolides in rat plasma after oral administration of Chansu extract by SPE-HPLC method.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,46(3),442-448.
MLA Liang, Yan,et al."Simultaneous determination and pharmacokinetics of five bufadienolides in rat plasma after oral administration of Chansu extract by SPE-HPLC method".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 46.3(2008):442-448.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。