LC-DAD analysis of six bioactive constituents in rat urine and bile after oral administration of luan-pao-prescription
文献类型：期刊论文
| 作者 | Zhang, Jin-Qiang1,2; Huang, Hui-Lian2; Chen, Guang-Tong1,2; Lu, Zhi-Qiang2; Tao, Si-Jia2; Yang, Min2; Sha, Na2; Li, Xing-Nuo1,2; Bi, Kai-Shun1; Guo, De-An2
|
| 刊名 | CHROMATOGRAPHIA
 |
| 出版日期 | 2008-02 |
| 卷号 | 67期号:3-4页码:325-329 |
| 关键词 | column liquid chromatography metabolism urinary and biliary excretion Luan-Pao-prescription |
| ISSN号 | 0009-5893 |
| DOI | 10.1365/s10337-007-0476-3 |
| 文献子类 | Article |
| 英文摘要 | A simple and sensitive high-performance liquid chromatographic method with diode-array detection has been developed and validated for determination of six bioactive constituents, baicalin (BG), wogonoside (WG), baicalein (BA), wogonin (WO), oroxylin A (OA), and rhein (RHE), in rat urine and bile after oral administration of Luan-Pao-Prescription. The separation was performed on a C-18 column at 30 degrees C with a mobile phase gradient prepared from 0.5% aqueous glacial acetic acid and acetonitrile. Detection was at 320 nm for BG, WG, BA, WO, and OA, and 254 nm for RHE, for both biological fluids. Because of the properties of the compounds of interest in urine and bile, two methods of sample preparation were used-solid phase extraction for monitoring BG and WG and liquid-liquid extraction for BA, WO, OA, and RHE. The HPLC method was validated for linearity, LOD, LLOQ, precision, accuracy, and recovery from the biological fluids in accordance with ICH guidelines. The method was successfully used to investigate urinary and biliary excretion of the six constituents in rats. |
| WOS关键词 | SOLID-PHASE EXTRACTION ; SCUTELLARIA-BAICALENSIS GEORGI ; CANCER CELL-LINES ; IN-VITRO ; LIQUID-CHROMATOGRAPHY ; BAICALIN ; WOGONIN ; RADIX ; METABOLITES ; PLASMA |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000252806200020 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272994]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Guo, De-An |
| 作者单位 | 1.Shenyang Pharmaceut Univ, Shenyang 110016, Liaoning, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhang, Jin-Qiang,Huang, Hui-Lian,Chen, Guang-Tong,et al. LC-DAD analysis of six bioactive constituents in rat urine and bile after oral administration of luan-pao-prescription[J]. CHROMATOGRAPHIA,2008,67(3-4):325-329. |
| APA | Zhang, Jin-Qiang.,Huang, Hui-Lian.,Chen, Guang-Tong.,Lu, Zhi-Qiang.,Tao, Si-Jia.,...&Guo, De-An.(2008).LC-DAD analysis of six bioactive constituents in rat urine and bile after oral administration of luan-pao-prescription.CHROMATOGRAPHIA,67(3-4),325-329. |
| MLA | Zhang, Jin-Qiang,et al."LC-DAD analysis of six bioactive constituents in rat urine and bile after oral administration of luan-pao-prescription".CHROMATOGRAPHIA 67.3-4(2008):325-329. |
入库方式： OAI收割
来源：上海药物研究所
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