Mechanism underlying the anti-angiogenetic effect of the novel tubulin inhibitor C9
文献类型：期刊论文
| 作者 | Ren Xuan; Lin Liping; Ding Jian
|
| 刊名 | Chinese Journal New Drugs
 |
| 出版日期 | 2011 |
| 卷号 | 20期号:17页码:1703-1711 |
| 关键词 | C9 C9 angiogenesis endothelial cell MAPK pathway |
| ISSN号 | 1003-3734 |
| 其他题名 | 微管抑制剂C9抑制新生血管生成的作用机 |
| 文献子类 | Article |
| 英文摘要 | Objective: To investigate mechanism underlying the anti-angiogenetic effect of C9, a new tubulin inhibitor.Methods:Antagonizing effect of C9 on anti-apoptotic activity of VEGF and bFGF was determined by FACS and TUNEL assay in endothelial cells cultured in serum-free media.Phosphorylation of c-Raf,Mek,Erk and Akt was detected by Western blot after treatment with various concentrations of C9.Results:FACS and TUNEL assay showed that C9 antagonized the anti-apoptosis activity of VEGF and bFGF on apoptosis induced by serum-free culture in endothelial cells.Western Blot revealed that C9 at various concentrations downregulated phosphorylation of c-Raf,Mek,Erk and Akt,but did not affect the phosphorylation of KDR or FGFR1.Conclusion:C9 inhibits the angiogenesis process by targeting the Raf-Mek-Erk-MAPK pathway. |
| 资助项目 | 国家自然科学基金[00000000] |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:4332067 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/267903]  |
| 专题 | 科研与新药推进处 |
| 通讯作者 | Lin Liping |
| 作者单位 | State Key Laboratory of Drug Research, Shanghai Institute of Materia Medidca,Chinese Academy of Sciences, Shanghai 201203, China. |
| 推荐引用方式 GB/T 7714 | Ren Xuan,Lin Liping,Ding Jian. Mechanism underlying the anti-angiogenetic effect of the novel tubulin inhibitor C9[J]. Chinese Journal New Drugs,2011,20(17):1703-1711. |
| APA | Ren Xuan,Lin Liping,&Ding Jian.(2011).Mechanism underlying the anti-angiogenetic effect of the novel tubulin inhibitor C9.Chinese Journal New Drugs,20(17),1703-1711. |
| MLA | Ren Xuan,et al."Mechanism underlying the anti-angiogenetic effect of the novel tubulin inhibitor C9".Chinese Journal New Drugs 20.17(2011):1703-1711. |
入库方式： OAI收割
来源：上海药物研究所
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