Analysis of triterpenoids in Ganoderma lucidum using liquid chromatography coupled with electrospray ionization mass spectrometry
文献类型：期刊论文
| 作者 | Yang, Min; Wang, Xiaoming; Guan, Shuhong ; Xia, Jiameng; Sun, Jianghao; Guo, Hui; Guo, De-an
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| 刊名 | JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
 |
| 出版日期 | 2007-05 |
| 卷号 | 18期号:5页码:927-939 |
| ISSN号 | 1044-0305 |
| DOI | 10.1016/j.jasms.2007.01.012 |
| 文献子类 | Article |
| 英文摘要 | Triterpenoids extracted from Ganoderma lucidum (Leyss. ex Fr.) Karst were separated and characterized using optimized reversed-phase liquid chromatography with diode array detection and electrospray ion trap tandem mass spectrometry (HPLC-DAD-ESI-MSn). They could be classified into five types depending on the fragmentation behavior. All triterpenoids gave [M - H](-) and [2M - H](-) ions by electrospray ionization monitored in the negative ion mode; in addition, compounds of types III and IV gave prominent [M - H - H2O](-) ions and the unsaturated bond at C-20, 22 would reduce the abundance of [M - H - H2O](-) ion. The key fragmentation information was cleavage at C- and D-rings despite the predominant losses of H2O and CO2. Compounds with hydroxyls at C-7 and C-15 would produce a list of b, b - 1, b - 2, and b - 16 ions attributed to cleavage of D-ring; if the second alcohol at C-15 were oxidized to ketone, the prominent cleavage would occur at C-ring and produce a group of ions of a; if C-7 were oxidized to ketone, transference of two hydrogen atoms would occur during the cleavage of rings and a list of ions about a + 2 and/or b + 2 would appear instead. The above fragmentations and regularities in fragmentation pathways were reported for the first time, and were implemented for the analysis of triterpenoids in G. lucidum. The chloroform extract was separated on a Zorbax SB-C-18 column, eluting with an acetonitrile-0.2% acetic acid gradient. A total of 32 triterpenoids, including six new ones, were identified or tentatively characterized based on the tandem mass spectra of the HPLC peaks. |
| WOS关键词 | INHIBITORY TRITERPENES ; FR KARST ; ACID ; EXTRACT ; SPORES ; CONSTITUENTS ; MECHANISMS ; TERPENOIDS ; CELLS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:000246237300014 |
| 出版者 | ELSEVIER SCIENCE INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273274]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Guo, De-an |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Res Ctr TCM Modernizat, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Min,Wang, Xiaoming,Guan, Shuhong,et al. Analysis of triterpenoids in Ganoderma lucidum using liquid chromatography coupled with electrospray ionization mass spectrometry[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,2007,18(5):927-939. |
| APA | Yang, Min.,Wang, Xiaoming.,Guan, Shuhong.,Xia, Jiameng.,Sun, Jianghao.,...&Guo, De-an.(2007).Analysis of triterpenoids in Ganoderma lucidum using liquid chromatography coupled with electrospray ionization mass spectrometry.JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY,18(5),927-939. |
| MLA | Yang, Min,et al."Analysis of triterpenoids in Ganoderma lucidum using liquid chromatography coupled with electrospray ionization mass spectrometry".JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY 18.5(2007):927-939. |
入库方式： OAI收割
来源：上海药物研究所
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