HPLC method for the determination and pharmacokinetic studies of four triterpenoids in rat plasma after oral administration of Ganoderma lucidum extract
文献类型：期刊论文
| 作者 | Wang, Xiaoming; Liu, Rongxia; Sun, Jianghao; Guan, Shuhong ; Yang, Min; Bi, Kaishun; Guo, Dean
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| 刊名 | BIOMEDICAL CHROMATOGRAPHY
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| 出版日期 | 2007-04 |
| 卷号 | 21期号:4页码:389-396 |
| 关键词 | Ganoderma lucidum triterpenoids HPLC pharmacokinetics |
| ISSN号 | 0269-3879 |
| DOI | 10.1002/bmc.768 |
| 文献子类 | Article |
| 英文摘要 | Four major triterpenoids (ganoderic acids C-2, B, K and H) in rat plasma after oral administration of G. lucidum extract were analyzed quantitatively by high-performance liquid chromatography (HPLC). Plasma samples taken from rats were acidified with hydrochloric acid and extracted with dichloromethane-ethyl acetate (90:10). The chromatographic separation was achieved on an Agilent Zorbax SB-C-18 column (250 x 4.6 mm, 5 mu m) at 35 degrees C, with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.0 mL/min. The four triterpenoids and internal standard (hydrocortisone) were detected at a wavelength 252 nm. All calibration curves showed good linearity (r(2) > 0.99) within test ranges. The relative deviation of this method was less than 10% for intra- and inter-day assays, and the accuracy ranged from 89 to 108%. The extract recovery for the four triterpenoids and internal standard ranged from 95 to 67%, and the QC samples were found to be stable according to the results of the stability study. This is the first report on determination of the major triterpenoids in rat plasma after oral administration of G. lucidum extract and the results provided a firm basis for clarifying the pharmacological effect of G. lucidum and evaluating the clinical applications of this medicinal fungus. Copyright (c) 2007 John Wiley & Sons, Ltd. |
| WOS关键词 | FR KARST ; INHIBITORY TRITERPENES ; CONSTITUENTS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000245692200010 |
| 出版者 | JOHN WILEY & SONS LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273290]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Guo, Dean |
| 作者单位 | 1.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China 2.Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai Res Ctr TCM Modernizat, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiaoming,Liu, Rongxia,Sun, Jianghao,et al. HPLC method for the determination and pharmacokinetic studies of four triterpenoids in rat plasma after oral administration of Ganoderma lucidum extract[J]. BIOMEDICAL CHROMATOGRAPHY,2007,21(4):389-396. |
| APA | Wang, Xiaoming.,Liu, Rongxia.,Sun, Jianghao.,Guan, Shuhong.,Yang, Min.,...&Guo, Dean.(2007).HPLC method for the determination and pharmacokinetic studies of four triterpenoids in rat plasma after oral administration of Ganoderma lucidum extract.BIOMEDICAL CHROMATOGRAPHY,21(4),389-396. |
| MLA | Wang, Xiaoming,et al."HPLC method for the determination and pharmacokinetic studies of four triterpenoids in rat plasma after oral administration of Ganoderma lucidum extract".BIOMEDICAL CHROMATOGRAPHY 21.4(2007):389-396. |
入库方式： OAI收割
来源：上海药物研究所
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