HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum
文献类型：期刊论文
| 作者 | Wang, Xiao-Ming; Guan, Shu-Hong ; Liu, Rong-Xia; Sun, Jiang-Hao; Liang, Yan; Yang, Min; Wang, Wei; Bi, Kai-Shun; Guo, De-An
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| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2007-02-19 |
| 卷号 | 43期号:3页码:1185-1190 |
| 关键词 | Ganoderma lucidum total triterpenoids rat urine HPLC |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2006.10.010 |
| 文献子类 | Article |
| 英文摘要 | A sensitive and simple high-performance liquid chromatography (HPLC) method was applied for the quantitative determination of four major triterpenoids (ganoderic acids C-2, B, K and H) in rat urine after oral administration of total triterpenoids from Ganoderma lucidum. The urine sample was extracted with dichloromethane-ethyl acetate (90: 10) after acidification by hydrochloric acid (0.2 mol/ml). Chromatographic separation was achieved on a Zorbax SB-C-18 column (250 mm x 4.6 mm, 5 mu m) at 35 degrees C, with a linear gradient of acetonitrile and 0.03% aqueous phosphoric acid (v/v), at a flow rate of 1.2 ml/min. The four triterpenoids and internal standard (hydrocortisone) were detected at a wavelength 252 nm. The within- and between-day assay coefficients of variation for the four triterpenoids in urine were less than 9% and the extraction recovery of this method was higher than 90%. Using this method, the excretion profile of the triterpenoids in rat urine after oral administration of total triterpenoids of G. lucidum was revealed for the first time. (c) 2006 Elsevier B.V. All rights reserved. |
| WOS关键词 | FR KARST ; ACID-F ; CELLS ; CONSTITUENTS |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000244623000056 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273322]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Guo, De-An |
| 作者单位 | 1.Shenyang Pharmaceut Univ, Shenyang 110016, Liaoning, Peoples R China 2.Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai Res Ctr TCM Modernizat, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xiao-Ming,Guan, Shu-Hong,Liu, Rong-Xia,et al. HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2007,43(3):1185-1190. |
| APA | Wang, Xiao-Ming.,Guan, Shu-Hong.,Liu, Rong-Xia.,Sun, Jiang-Hao.,Liang, Yan.,...&Guo, De-An.(2007).HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,43(3),1185-1190. |
| MLA | Wang, Xiao-Ming,et al."HPLC determination of four triterpenoids in rat urine after oral administration of total triterpenoids from Ganoderma lucidum".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 43.3(2007):1185-1190. |
入库方式： OAI收割
来源：上海药物研究所
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