Large-scale isolation and purification of scoparone from Herba artemisiae scopariae by high-speed counter-current chromatography
文献类型：期刊论文
| 作者 | Ma, C. H.; Ke, W.; Sun, Z. L.; Peng, J. Y.; Li, Z. X.; Zhou, X.; Fan, G. R.; Huang, C. G. |
| 刊名 | CHROMATOGRAPHIA
 |
| 出版日期 | 2006-07 |
| 卷号 | 64期号:1-2页码:83-87 |
| 关键词 | column liquid chromatography counter-current chromatography coumarin compounds scoparone Herba artemisiae scopariae Artemisia capillaries and Artemisia scoparia |
| ISSN号 | 0009-5893 |
| DOI | 10.1365/s10337-006-0789-7 |
| 文献子类 | Article |
| 英文摘要 | A preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol- water (1:1:0.45:1.55, v/v/v/v) was successfully performed to isolate scoparone (6,7-dimethoxycoumarin, 6,7-dimethylesculetin, 6,7-DME) from the plant of Herba artemisiae scopariae, a traditional Chinese medicine. 233.5 mg Scoparone with the purity of 96.8% (determined by HPLC) was obtained in one-step elution from 800 mg crude extract. The recovery of scoparone was 91.8%, and the chemical structure of this compound was identified by IR, MS, H-1 NMR and C-13 NMR spectrum. |
| WOS关键词 | CONSTITUENT ; SEPARATION ; COUMARINS |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000239677700013 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273552]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Fan, G. R. |
| 作者单位 | 1.Harbin Univ Commerce, Ctr Res & Dev Life Sci & Environm Sci, Harbin 150076, Peoples R China 2.Second Mil Med Univ, Sch Pharm, Shanghai Key Lab Pharmaceut Metabolite Res, Shanghai 200433, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, C. H.,Ke, W.,Sun, Z. L.,et al. Large-scale isolation and purification of scoparone from Herba artemisiae scopariae by high-speed counter-current chromatography[J]. CHROMATOGRAPHIA,2006,64(1-2):83-87. |
| APA | Ma, C. H..,Ke, W..,Sun, Z. L..,Peng, J. Y..,Li, Z. X..,...&Huang, C. G..(2006).Large-scale isolation and purification of scoparone from Herba artemisiae scopariae by high-speed counter-current chromatography.CHROMATOGRAPHIA,64(1-2),83-87. |
| MLA | Ma, C. H.,et al."Large-scale isolation and purification of scoparone from Herba artemisiae scopariae by high-speed counter-current chromatography".CHROMATOGRAPHIA 64.1-2(2006):83-87. |
入库方式： OAI收割
来源：上海药物研究所
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