Identification and determination of major flavonoids in rat serum by HPLC-UV and HPLC-MS methods following oral administration of Dalbergia odorifera extract
文献类型：期刊论文
| 作者 | Liu, RX; Sun, JH ; Bi, KS; Guo, DA
|
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2005-12-27 |
| 卷号 | 829期号:1-2页码:35-44 |
| 关键词 | Dalbergia odorifera flavonoids (3R)-4'-methoxy-2',3,7-trihydroxy-isoflavanone vestitone formononetin sativanone |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2005.09.025 |
| 文献子类 | Article |
| 英文摘要 | The major flavonoids in rat serum after oral administration of Dalbergia odorifera extract were analyzed qualitatively and quantitatively by high performance liquid chromatography (HPLC) and its coupling to mass spectrometry (HPLC-C-MS). Utilizing HPLC-MS technique, 18 flavonoids including five isoflavones, four isoflavanones, four neoflavones, two flavanones, two chalcones, one isoflavanonol were identified in free form in serum sample based on comparison with the authentic standards. Furthermore, the amounts of the four prominent flavonoids, (3R)-4'-methoxy2',3,7-trihydroxyisoflavanone, vestitone, formononetin and sativanone were determined in serum by HPLC-UV with internal standard method. The method was validated and utilized in phannacokinetic studies of these four analytes. This is the first report on identification and determination of the major flavonoids in rat serum after oral administration of D. odorifera extract and the results provided a firm basis for clarifying the pharmacological effect of D. odorifera and evaluating the clinical applications of this medicinal herb. (c) 2005 Elsevier B.V. All rights reserved. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; HUMAN URINE ; MASS-SPECTROMETRY ; HUMAN PLASMA ; METABOLITES ; APOPTOSIS ; BUTEIN ; CELLS ; FORMONONETIN ; ISOFLAVONES |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000234158800003 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/273753]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Guo, DA |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai Res Ctr TCM Modernizat, Shanghai 201203, Peoples R China 2.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, RX,Sun, JH,Bi, KS,et al. Identification and determination of major flavonoids in rat serum by HPLC-UV and HPLC-MS methods following oral administration of Dalbergia odorifera extract[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2005,829(1-2):35-44. |
| APA | Liu, RX,Sun, JH,Bi, KS,&Guo, DA.(2005).Identification and determination of major flavonoids in rat serum by HPLC-UV and HPLC-MS methods following oral administration of Dalbergia odorifera extract.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,829(1-2),35-44. |
| MLA | Liu, RX,et al."Identification and determination of major flavonoids in rat serum by HPLC-UV and HPLC-MS methods following oral administration of Dalbergia odorifera extract".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 829.1-2(2005):35-44. |
入库方式： OAI收割
来源：上海药物研究所
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