Colocalization of phosphorylated CREB with calcium/calmodulin-dependent protein kinase IV in hippocampal neurons induced by ohmfentanyl stereoisomers
文献类型：期刊论文
| 作者 | Gao, C; Chen, LW; Tao, YM; Chen, J ; Xu, XJ; Zhang, GY; Chi, ZQ
|
| 刊名 | BRAIN RESEARCH
 |
| 出版日期 | 2004-10-22 |
| 卷号 | 1024期号:1-2页码:25-33 |
| 关键词 | ohmfentanyl stereoisomer cAMP response element-binding protein Ca2+/calmodulin-dependent protein kinase IV hippocampal neuron opioids dependence phosphorylation |
| ISSN号 | 0006-8993 |
| DOI | 10.1016/j.brainres.2004.06.084 |
| 文献子类 | Article |
| 英文摘要 | The transcription factor cAMP response element-binding protein (CREB) plays an important role in opioids dependence. To better understand the role of CREB in opioids dependence and underlying signal pathways, we compared the effects of three ohmfentanyl stereoisomers ((-)-cis-(3R,4S,2' R) OMF (179202), (+)-cis-(3R,4S,2' S) OMF (179204), (-)-cis-(3S,4S,2' R) OMF (179203)) and morphine on CREB phosphorylation and the expression of Ca2+/calmodulin-dependent protein kinase IV (CaMKIV) in hippocampus derived from mice which displayed conditioned place preference (CPP) behavior by Western blot, and immunohistochemistry analyses. Moreover, we studied the effects of OMF and morphine on CREB phosphorylation and colocalization of phosphorylated CREB (P-CREB) with CaMKIV in cultured rat hippocampal neurons by Western blot, and confocal fluorescence microscopy analyses. The results showed that 179202, 179204 or morphine, which could induce CPP, enhanced CREB phosphorylation and the expression of CaMKIV in hippocampus from CPP mice without affecting total CREB protein level. The CREB phosphorylation of cultured hippocampal neurons was also enhanced and reached its peak level at 30 min upon exposure to 179202 (100 nM), 179204 (100 nM) or morphine (1 muM), while the total CREB protein level was not altered. KN-62 (10 muM), an inhibitor of CaM kinases, prevented CREB phosphorylation induced by morphine, 179202, and 179204 without change of total CREB level. The results of confocal fluorescence microscopy further demonstrated that the activated CREB (P-CREB) was colocalized with CaMKIV in nucleus. 179203, which could not induce CPP, failed to increase the CREB phosphorylation and the colocalization of P-CREB with CaMKIV both in hippocampus from CPP mice and in cultured hippocampal neurons. This is the first evidence to suggest that the increased CREB phosphorylation via CaMKIV signal pathway in hippocampus is relevant to opioids psychological dependence. (C) 2004 Elsevier B.V. All rights reserved. |
| WOS关键词 | ELEMENT-BINDING PROTEIN ; OHMEFENTANYL STEREOISOMERS ; ANALGESIC ACTIVITY ; OPIOID RECEPTORS ; CHRONIC MORPHINE ; RAT HIPPOCAMPUS ; GENE-EXPRESSION ; ADDICTION ; MECHANISMS ; MICE |
| WOS研究方向 | Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:000224664800003 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274003]  |
| 专题 | 院士及顾问专家 |
| 通讯作者 | Chi, ZQ |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Xuzhou Med Coll, Res Ctr Biochem & Mol Biol, Xuzhou 221002, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, C,Chen, LW,Tao, YM,et al. Colocalization of phosphorylated CREB with calcium/calmodulin-dependent protein kinase IV in hippocampal neurons induced by ohmfentanyl stereoisomers[J]. BRAIN RESEARCH,2004,1024(1-2):25-33. |
| APA | Gao, C.,Chen, LW.,Tao, YM.,Chen, J.,Xu, XJ.,...&Chi, ZQ.(2004).Colocalization of phosphorylated CREB with calcium/calmodulin-dependent protein kinase IV in hippocampal neurons induced by ohmfentanyl stereoisomers.BRAIN RESEARCH,1024(1-2),25-33. |
| MLA | Gao, C,et al."Colocalization of phosphorylated CREB with calcium/calmodulin-dependent protein kinase IV in hippocampal neurons induced by ohmfentanyl stereoisomers".BRAIN RESEARCH 1024.1-2(2004):25-33. |
入库方式： OAI收割
来源：上海药物研究所
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