Antiangiogenic effect of alpha-anordrin in vitro and in vivo
文献类型：期刊论文
| 作者 | Ma, ZC; Lou, LG ; Zhang, Z; Xu, B
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2000-10 |
| 卷号 | 21期号:10页码:939-944 |
| 关键词 | alpha-anordrin angiogenesis factor choriocarcinoma umbilical veins vascular endothelium nitric oxide |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: To study the antiangiogenic effect of alpha-anordrin (alpha-Ano), a partial. antagonist of estrogen receptor. METHODS: The in vivo inhibitory effect of alpha-Ano on angiogenesis was determined by microvascular density (MVD) in tumors and the chicken chorioallantoic membrane (CAM) model. The in vitro effects of alpha-Ano on proliferation, migration, and attachment of human umbilical vein endothelial cells (HUVEC) were assessed by trypan blue exclusion, wound-induced two-dimensional migration model, and their ability to adhere to type I collagen, respectively. The possible involvement of nitric oxide (NO) in alpha-Ano antiangiogenic effect was determined by measuring NO content using fluorescent assay. RESULTS: alpha-Ano significantly inhibited the MVD in Lewis lung carcinoma model and this effect was correlated with its inhibition of the tumor growth. alpha-Ano also showed an inhibitory effect on the angiogenesis of CAM with the inhibitory rate of 53 % and such action of alpha-Ano could not be blocked by simultaneous administration of 17 beta-estrodiol, a typical agonist of estrogen receptor. In vitro studies showed that alpha-ANO obviously suppressed the proliferation and migration of HUVEC, but had no obvious effect on the attachment of HUVEC to the type I collagen. Moreover, alpha-Ano significantly reduced the level of NO released by HUVEC in a dose- and time-dependent manner. CONCLUSION: alpha-Ano possesses an antiangiogenic effect, and this effect is mediated, at least in part, by reducing the NO content and subsequently inhibiting the proliferation and migration of endothelial cells. |
| WOS关键词 | TUMOR ANGIOGENESIS ; GROWTH FACTOR ; NITRIC-OXIDE |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000089725800015 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274570]  |
| 专题 | 院士及顾问专家 药物安全性评价中心 |
| 通讯作者 | Xu, B |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, ZC,Lou, LG,Zhang, Z,et al. Antiangiogenic effect of alpha-anordrin in vitro and in vivo[J]. ACTA PHARMACOLOGICA SINICA,2000,21(10):939-944. |
| APA | Ma, ZC,Lou, LG,Zhang, Z,&Xu, B.(2000).Antiangiogenic effect of alpha-anordrin in vitro and in vivo.ACTA PHARMACOLOGICA SINICA,21(10),939-944. |
| MLA | Ma, ZC,et al."Antiangiogenic effect of alpha-anordrin in vitro and in vivo".ACTA PHARMACOLOGICA SINICA 21.10(2000):939-944. |
入库方式： OAI收割
来源：上海药物研究所
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