Differential regulation of P53, c-Myc, Bcl-2, Bax and AFP protein expression, and caspase activity during 10-hydroxycamptothecin-induced apoptosis in Hep G2 cells
文献类型：期刊论文
| 作者 | Zhang, XW; Xu, B
|
| 刊名 | ANTI-CANCER DRUGS
 |
| 出版日期 | 2000-10 |
| 卷号 | 11期号:9页码:747-756 |
| 关键词 | apoptosis caspases gene expression Hep G2 cell line 10-hydroxycamptothecin |
| ISSN号 | 0959-4973 |
| DOI | 10.1097/00001813-200010000-00012 |
| 文献子类 | Article |
| 英文摘要 | 10-Hydroxycamptothecin (HCPT), a DNA topoisomerase I (Topo I) inhibitor, exhibited a remarkable apoptosis-inducing effect on human hepatoma Hep G2 cells. We studied the effect of HCPT upon the expression of P53, c-Myc, Bcl-2, Bax and a-fetoprotein (AFP) proteins, and caspase (caspase-1 and caspase-3) activity of Hep G2 cells. It showed that HCPT at a dose of 0.1 mug/ml increased the expression of P53, c-Myc and Bax protein, and decreased the expression of Bcl-2 and AFP. The increase of P53, which was remarkable after only 3 h incubation with HCPT, occurred much earlier than the changes of other proteins, suggesting that the increase of P53 expression may be the upstream event in the apoptosis of Hep G2 cells induced by HCPT. Both caspase-1 and caspase-3 were activated in Hep G2 cells by HCPT treatment, suggesting that caspase-1 and caspase-3 are involved in the process of apoptosis in Hep G2 cells, and may be the main effecters of the apoptosis. [(C) 2000 Lippincott Williams & Wilkins]. |
| WOS关键词 | WILD-TYPE P53 ; MEDIATED APOPTOSIS ; IN-VIVO ; DEATH ; INDUCTION ; FAMILY ; ACTIVATION ; ARREST ; AGENTS |
| WOS研究方向 | Oncology ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000165667100012 |
| 出版者 | LIPPINCOTT WILLIAMS & WILKINS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274571]  |
| 专题 | 院士及顾问专家 药物安全性评价中心 国家新药筛选中心 |
| 通讯作者 | Xu, B |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Div Canc Pharmacol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, XW,Xu, B. Differential regulation of P53, c-Myc, Bcl-2, Bax and AFP protein expression, and caspase activity during 10-hydroxycamptothecin-induced apoptosis in Hep G2 cells[J]. ANTI-CANCER DRUGS,2000,11(9):747-756. |
| APA | Zhang, XW,&Xu, B.(2000).Differential regulation of P53, c-Myc, Bcl-2, Bax and AFP protein expression, and caspase activity during 10-hydroxycamptothecin-induced apoptosis in Hep G2 cells.ANTI-CANCER DRUGS,11(9),747-756. |
| MLA | Zhang, XW,et al."Differential regulation of P53, c-Myc, Bcl-2, Bax and AFP protein expression, and caspase activity during 10-hydroxycamptothecin-induced apoptosis in Hep G2 cells".ANTI-CANCER DRUGS 11.9(2000):747-756. |
入库方式： OAI收割
来源：上海药物研究所
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