Differentiation-inducing action of 10-hydroxycamptothecian on human hepatoma Hep G2 cells
文献类型：期刊论文
| 作者 | Zhang, XW; Jiang, JF; Xu, B
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2000-04 |
| 卷号 | 21期号:4页码:364-368 |
| 关键词 | 10-hydroxycamptothecin experimental liver neoplasm cell differentiation cell cycle protein p53 immunohistochemistry proliferating cell nuclear antigens telomerase flow cytometry |
| ISSN号 | 0253-9756 |
| 文献子类 | Article |
| 英文摘要 | AIM: To study the mechanism of differentiation-inducing action of 10-hydroxycamptothecin (HCPT) on human hepatoma Hep G2 cells, METHODS: The proliferating cell nuclear antigen (PCNA) expression was studied by immunocytochemical staining method. The cell cycle distribution and the wild-type protein p53 expression were measured by flow cytometry. Telomerase activity was assayed with telomeric repeat amplification protocol (TRAP). RESULTS: After treatment with HCPT at differentiation-inducing concentrations 5 - 20 mu g.L-1 for 6 d, Hep G2 cells were mainly arrested at G2/M phase and the PCNA expression rate was lower than that of control cells. When Hep G2 cells grew in a medium containing HCPT 5 mu g.L-1 for 6 d, the p53 expression level markedly increased in comparison with the control cells. The telomerase activity did not change in Hep G2 cells treated with HCPT 5 - 20 mu g.L-1 for 8 d. CONCLUSION: The differentiation-inducing effect of HCPT on Hep G2 cells is related with the cell cycle arrest at G(2)/M phase, down-regulation of PCNA and up-regulation of wild-type protein p53. |
| WOS关键词 | TELOMERASE ACTIVITY ; PROTEIN ; K562 |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000086478800018 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/274629]  |
| 专题 | 院士及顾问专家 药物安全性评价中心 国家新药筛选中心 |
| 通讯作者 | Xu, B |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Dept Pharmacol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, XW,Jiang, JF,Xu, B. Differentiation-inducing action of 10-hydroxycamptothecian on human hepatoma Hep G2 cells[J]. ACTA PHARMACOLOGICA SINICA,2000,21(4):364-368. |
| APA | Zhang, XW,Jiang, JF,&Xu, B.(2000).Differentiation-inducing action of 10-hydroxycamptothecian on human hepatoma Hep G2 cells.ACTA PHARMACOLOGICA SINICA,21(4),364-368. |
| MLA | Zhang, XW,et al."Differentiation-inducing action of 10-hydroxycamptothecian on human hepatoma Hep G2 cells".ACTA PHARMACOLOGICA SINICA 21.4(2000):364-368. |
入库方式： OAI收割
来源：上海药物研究所
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