The profiling of the metabolites of hirsutine in rat by ultra-high performance liquid chromatography coupled with linear ion trap Orbitrap mass spectrometry: An improved strategy for the systematic screening and identification of metabolites in multi-samples in vivo
文献类型：期刊论文
| 作者 | Wang, Jianwei1,2,3; Qi, Peng2; Hou, Jinjun2 ; Shen, Yao2; Yang, Min2; Bi, Qirui1,2; Deng, Yanping2; Shi, Xiaojian1,2; Feng, Ruihong2; Feng, Zijin2
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| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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| 出版日期 | 2017-02-05 |
| 卷号 | 134页码:149-157 |
| 关键词 | Hirsutine Metabolic profile Multi-samples Metabolites systematic screening Uncaria rhynchophylla UHPLC/LTQ-Orbitrap-MS |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2016.11.034 |
| 文献子类 | Article |
| 英文摘要 | Drug metabolites identification and construction of metabolic profile are meaningful work for the drug discovery and development. The great challenge during this process is the work of the structural clarification of possible metabolites in the complicated biological matrix, which often resulting in a huge amount data sets, especially in multi-samples in vivo. Analyzing these complex data manually is time-consuming and laborious. The object of this study was to develop a practical strategy for screening and identifying of metabolites from multiple biological samples efficiently. Using hirsutine (HTI), an active components of Uncaria rhynchophylla (Gouteng in Chinese) as a model and its plasma, urine, bile, feces and various tissues were analyzed with data processing software (Metwork), data mining tool (Progenesis QI), and HR-MSn data by ultra-high performance liquid chromatography/linear ion trap-Orbitrap mass spectrometry (U-HPLC/LTQ-Orbitrap-MS). A total of 67 metabolites of HTI in rat biological samples were tentatively identified with established library, and to our knowledge most of which were reported for the first time. The possible metabolic pathways were subsequently proposed, hydroxylation, dehydrogenation, oxidation, N-oxidation, hydrolysis, reduction and glucuronide conjugation were mainly involved according to metabolic profile. The result proved application of this improved strategy was efficient, rapid, and reliable for metabolic profiling of components in multiple biological samples and could significantly expand our understanding of metabolic situation of TCM in vivo. (C) 2016 Elsevier B.V. All rights reserved. |
| WOS关键词 | HUMAN INTESTINAL MICROFLORA ; UNCARIA-RHYNCHOPHYLLA ; TISSUE DISTRIBUTION ; INDOLE ALKALOIDS ; SMALL MOLECULES ; DRUG DISCOVERY ; DISPOSITION ; RESOLUTION ; BIOTRANSFORMATION ; METABOLOMICS |
| 资助项目 | National Natural Science Foundation of China[81403097] ; National Natural Science Foundation of China[81530095] ; National Science and Technology Major Project for Major Drug Development[2014ZX09304-307-001-007] ; Special Scientific Research in the Chinese Medicine Industry of State Administration of Traditional Chinese Medicine[201307002] ; Jiangsu Overseas Research &Training Program for University Prominent Young &Middle-aged Teachers and President[00000000] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000392909900020 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275634]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Wu, Wanying; Guo, Dean |
| 作者单位 | 1.China Pharmaceut Univ, Coll Tradit Chinese Med, Nanjing 210009, Jiangsu, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Haike Rd 501, Shanghai 201203, Peoples R China; 3.Nantong Vocat Univ, Sch Chem & Biol Engn, Nantong 226007, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jianwei,Qi, Peng,Hou, Jinjun,et al. The profiling of the metabolites of hirsutine in rat by ultra-high performance liquid chromatography coupled with linear ion trap Orbitrap mass spectrometry: An improved strategy for the systematic screening and identification of metabolites in multi-samples in vivo[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2017,134:149-157. |
| APA | Wang, Jianwei.,Qi, Peng.,Hou, Jinjun.,Shen, Yao.,Yang, Min.,...&Guo, Dean.(2017).The profiling of the metabolites of hirsutine in rat by ultra-high performance liquid chromatography coupled with linear ion trap Orbitrap mass spectrometry: An improved strategy for the systematic screening and identification of metabolites in multi-samples in vivo.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,134,149-157. |
| MLA | Wang, Jianwei,et al."The profiling of the metabolites of hirsutine in rat by ultra-high performance liquid chromatography coupled with linear ion trap Orbitrap mass spectrometry: An improved strategy for the systematic screening and identification of metabolites in multi-samples in vivo".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 134(2017):149-157. |
入库方式： OAI收割
来源：上海药物研究所
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