UHPLC-Q-TOF-MS-based metabolomics approach to compare the saponin compositions of Xueshuantong injection and Xuesaitong injection
文献类型：期刊论文
| 作者 | Yao, Changliang2,3; Yang, Wenzhi3; Zhang, Jingxian3; Qiu, Shi3; Chen, Ming1; Shi, Xiaojian3; Pan, Huiqin2,3; Wu, Wanying3 ; Guo, Dean3
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| 刊名 | JOURNAL OF SEPARATION SCIENCE
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| 出版日期 | 2017-02 |
| 卷号 | 40期号:4页码:834-841 |
| 关键词 | metabolomics notoginseng saponins traditional Chinese medicine Xuesaitong Xueshuantong |
| ISSN号 | 1615-9306 |
| DOI | 10.1002/jssc.201601122 |
| 文献子类 | Article |
| 英文摘要 | Various traditional Chinese medicine preparations developed from Notoginseng total saponins, including Xueshuantong injection and Xuesaitong injection, are extensively used in China to treat cardiocerebrovascular diseases. However, the difference of their saponin compositions remains unknown. An ultra high performance liquid chromatography with quadrupole time-of-flight mass spectrometry based metabolomics approach was developed to probe the saponin discrimination between Xueshuantong and Xuesaitong and the related factors by large sample analysis. A highly efficient chromatographic separation was achieved on an HSS T3 column within 20 min with the holistic metabolites information recorded in the negative MSE mode. A six-step data pretreatment procedure mainly based on Progenesis QI and mass defect filtering was established. Pattern recognition chemometrics was used to discover the potential saponin markers. The saponin composition of Wuzhou Xueshuantong showed distinct discrimination from the other products. Wuzhou Xueshuantong contains more abundant protopanaxatriol-type noto-R-1, Rg(1), Re, and protopanaxadiol-type Rb-1, but less Rd and other low-polarity protopanaxadiol-type ginsenosides. These differences could not directly correlate to the use of different parts of Panax notoginseng, but possibly to the different preparation techniques employed by different manufacturers. These results are beneficial to the establishment of pharmacopoeia standards and the assessment of the efficacy and adverse drug reactions for these homologous products. |
| WOS关键词 | MASS-SPECTROMETRY ; LIQUID-CHROMATOGRAPHY ; PANAX-GINSENG ; STRATEGY |
| 资助项目 | 56th China Post-doctoral Science Foundation[2014M560364] ; National Natural Science Foundation of China[81503240] ; National Natural Science Foundation of China[81473344] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000397828000002 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275640]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Wu, Wanying |
| 作者单位 | 1.Guangxi Wuzhou Pharmaceut Grp Co Ltd, Wuzhou, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Yao, Changliang,Yang, Wenzhi,Zhang, Jingxian,et al. UHPLC-Q-TOF-MS-based metabolomics approach to compare the saponin compositions of Xueshuantong injection and Xuesaitong injection[J]. JOURNAL OF SEPARATION SCIENCE,2017,40(4):834-841. |
| APA | Yao, Changliang.,Yang, Wenzhi.,Zhang, Jingxian.,Qiu, Shi.,Chen, Ming.,...&Guo, Dean.(2017).UHPLC-Q-TOF-MS-based metabolomics approach to compare the saponin compositions of Xueshuantong injection and Xuesaitong injection.JOURNAL OF SEPARATION SCIENCE,40(4),834-841. |
| MLA | Yao, Changliang,et al."UHPLC-Q-TOF-MS-based metabolomics approach to compare the saponin compositions of Xueshuantong injection and Xuesaitong injection".JOURNAL OF SEPARATION SCIENCE 40.4(2017):834-841. |
入库方式： OAI收割
来源：上海药物研究所
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