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Identification and differentiation of Panax ginseng, Panax quinquefolium, and Panax notoginseng by monitoring multiple diagnostic chemical markers
文献类型：期刊论文
| 作者 | Yang, Wenzhi2,3; Qiao, Xue3; Li, Kai3; Fan, Jingran3; Bo, Tao1; Guo, De-an2 ; Ye, Min3
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| 刊名 | ACTA PHARMACEUTICA SINICA B
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| 出版日期 | 2016-11 |
| 卷号 | 6期号:6页码:568-575 |
| 关键词 | Panax species Ginsenoside LC-MS fingerprinting Chemical marker TCM compound preparation |
| ISSN号 | 2211-3835 |
| DOI | 10.1016/j.apsb.2016.05.005 |
| 文献子类 | Article |
| 英文摘要 | To differentiate traditional Chinese medicines (TCM) derived from congeneric species in TCM compound preparations is usually challenging. The roots of Panax ginseng (PG), Panax quinquefolium (PQ) and Panax notoginseng (PN) are used as popular TCM. They contain similar triterpenoid saponins (ginsenosides) as the major bioactive constituents. Thus far, only a few chemical markers have been discovered to differentiate these three species. Herein we present a multiple marker detection approach to effectively differentiate the three Panax species, and to identify them in compound preparations. Firstly, 85 batches of crude drug samples (including 32 PG, 30 PQ, and 23 PN) were analyzed by monitoring 40 major ginsenosides in the extracted ion chromatograms (EICs) using a validated LC MS fingerprinting method. Secondly, the samples were clustered into different groups by pattern recognition chemometric approaches using PLS-DA and OPLS-DA models, and 17 diagnostic chemical markers were discovered. Aside from the previously known Rf and p-F11, ginsenoside Rsi could be a new marker to differentiate PG from PQ. Finally, the above multiple chemical markers were used to identify the Panax species in 60 batches of TCM compound preparations. (C) 2016 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. This is an open access article under the CC |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; NMR-BASED METABOLOMICS ; AMERICAN GINSENG ; ASIAN GINSENG ; GINSENOSIDES ; MEDICINE ; SAPONINS ; DISCRIMINATION ; PRODUCTS |
| 资助项目 | National Natural Science Foundation of China[81222054] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5846275 |
| WOS记录号 | WOS:000390869400008 |
| 出版者 | INST MATERIA MEDICA, CHINESE ACAD MEDICAL SCIENCES |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275825]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Guo, De-an; Ye, Min |
| 作者单位 | 1.Agilent Technol, Beijing 100102, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China; 3.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Yang, Wenzhi,Qiao, Xue,Li, Kai,et al. Identification and differentiation of Panax ginseng, Panax quinquefolium, and Panax notoginseng by monitoring multiple diagnostic chemical markers[J]. ACTA PHARMACEUTICA SINICA B,2016,6(6):568-575. |
| APA | Yang, Wenzhi.,Qiao, Xue.,Li, Kai.,Fan, Jingran.,Bo, Tao.,...&Ye, Min.(2016).Identification and differentiation of Panax ginseng, Panax quinquefolium, and Panax notoginseng by monitoring multiple diagnostic chemical markers.ACTA PHARMACEUTICA SINICA B,6(6),568-575. |
| MLA | Yang, Wenzhi,et al."Identification and differentiation of Panax ginseng, Panax quinquefolium, and Panax notoginseng by monitoring multiple diagnostic chemical markers".ACTA PHARMACEUTICA SINICA B 6.6(2016):568-575. |
入库方式： OAI收割
来源：上海药物研究所
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