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Profiling and identification of metabolites of isorhynchophylline in rats by ultra high performance liquid chromatography and linear ion trap Orbitrap mass spectrometry

文献类型：期刊论文

作者Wang, Jianwei1,2,3; Qi, Peng2; Hou, Jinjun2; Shen, Yao2; Yan, Bingpeng2,3; Bi, Qirui2,3; Feng, RuiHong2; Shi, Xiaojian2,3; Yang, Min2; Wu, Wanying2
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
出版日期2016-10-15
卷号1033页码:147-156
关键词Isorhynchophyllin UHPLC/LTQ-Orbitrap-MS Metabolic profile Metabolites
ISSN号1570-0232
DOI10.1016/j.jchromb.2016.08.013
文献子类Article
英文摘要The searching of potentially bioactive metabolites in the biological body is an interesting and meaningful work for the drug study. However, the structural clarification of possible metabolites is one of the most challenging tasks in drug metabolism studies because of the variety of metabolic reactions and complexity of metabolites in vivo. Here, an ultra high performance liquid chromatography/linear ion trap-Orbitrap mass spectrometry (U-HPLC/LTQ-Orbitrap-MS) with combination of data post-processing techniques, including extracted ion chromatogram (EIC) and multiple mass defect filters (MMDF), was established for profiling and identification of metabolites of isorhynchophylline (IR) in vivo and in vitro, and the possible metabolic pathways were subsequently proposed after the oral dose of 20 mg/kg; A total of 47 metabolites of IR were tentatively identified, including 47, 21, 18, and 25 metabolites in rat urine, plasma, liver and rat liver microsomes (RLM) samples, respectively. To our knowledge, most of them were reported for the first time. Seven metabolic pathways, including dehydrogenation, oxidation, hydrolysis, reduction, demethylation, hydroxylation and glucuronide conjugation were involved in the metabolism. Among them, dehydrogenation, hydrolysis, hydroxylation and oxidation were considered as the main metabolic pathway of metabolism according to metabolic profile of in vivo and in vitro. The relative percentage of each metabolite and main metabolite types were also determined to better understand the metabolic behavior of IR in rats. The newly discovered IR metabolites significantly expanded our understanding and were going to be greatly helpful for the further pharmacokinetic study of IR in vivo. (C) 2016 Elsevier B.V. All rights reserved.
WOS关键词HT22 CELL ASSAY ; UNCARIA-RHYNCHOPHYLLA ; NEUROPROTECTIVE ACTIVITIES ; INDOLE ALKALOIDS ; MS
资助项目National Natural Science Foundation of China[81073026] ; National Science and Technology Major Project for Major Drug Development[2013ZX09508104] ; National Science and Technology Major Project for Major Drug Development[2014ZX09304-307-001-007] ; Chinese Academy of Sciences China[KSZD-EWZ-004-01] ; Special Scientific Research in the Chinese Medicine Industry of State Administration of Traditional Chinese Medicine[201307002] ; Jiangsu Overseas Research & Training Program[00000000]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000385322600018
出版者ELSEVIER SCIENCE BV
源URL[http://119.78.100.183/handle/2S10ELR8/275856]  
专题上海中药现代化研究中心
通讯作者Wu, Wanying; Guo, De-an
作者单位1.Nantong Vocat Univ, Sch Chem & Biol Engn, Nantong 226007, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Haike Rd 501, Shanghai 201203, Peoples R China;
3.China Pharmaceut Univ, Coll Tradit Chinese Med, Nanjing 210009, Jiangsu, Peoples R China;
推荐引用方式
GB/T 7714		 Wang, Jianwei,Qi, Peng,Hou, Jinjun,et al. Profiling and identification of metabolites of isorhynchophylline in rats by ultra high performance liquid chromatography and linear ion trap Orbitrap mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2016,1033:147-156.
APA Wang, Jianwei.,Qi, Peng.,Hou, Jinjun.,Shen, Yao.,Yan, Bingpeng.,...&Guo, De-an.(2016).Profiling and identification of metabolites of isorhynchophylline in rats by ultra high performance liquid chromatography and linear ion trap Orbitrap mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1033,147-156.
MLA Wang, Jianwei,et al."Profiling and identification of metabolites of isorhynchophylline in rats by ultra high performance liquid chromatography and linear ion trap Orbitrap mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1033(2016):147-156.

入库方式： OAI收割

来源：上海药物研究所

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