Method development and application of offline two-dimensional liquid chromatography/quadrupole time-of-flight mass spectrometry-fast data directed analysis for comprehensive characterization of the saponins from Xueshuantong Injection
文献类型：期刊论文
| 作者 | Yang, Wenzhi1; Zhang, Jingxian1; Yao, Changliang1; Qiu, Shi1; Chen, Ming2; Pan, Huiqin1; Shi, Xiaojian1; Wu, Wanying1 ; Guo, Dean1
|
| 刊名 | JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
 |
| 出版日期 | 2016-09-05 |
| 卷号 | 128页码:322-332 |
| 关键词 | Xueshuantong Injection Minor saponins Offline two-dimensional liquid chromatography Quadrupole time-of-flight mass spectrometry Fast data directed analysis Structural elucidation |
| ISSN号 | 0731-7085 |
| DOI | 10.1016/j.jpba.2016.05.035 |
| 文献子类 | Article |
| 英文摘要 | Xueshuantong Injection (XSTI), derived from Notoginseng total saponins, is a popular traditional Chinese medicine injection for the treatment of thrombus-resultant diseases. Current knowledge on its therapeutic basis is limited to five major saponins, whereas those minor ones are rarely investigated. We herein develop an offline two-dimensional liquid chromatography/quadrupole time-of-flight mass spectrometry-fast data directed analysis (offline 2D LC/QTOF-Fast DDA) approach to systematically characterize the saponins contained in XSTI. Key parameters affecting chromatographic separation in 2D LC (including stationary phase, mobile phase, column temperature, and gradient elution program) and the detection by QTOF MS (involving spray voltage, cone voltage, and ramp collision energy) were optimized in sequence. The configured offline 2D LC system showed an orthogonality of 0.84 and a theoretical peak capacity of 8976. Total saponins in XSTI were fractionated into eleven samples by the first-dimensional hydrophilic interaction chromatography, which were further analyzed by reversed phase UHPLC/QTOF-Fast DDA in negative ion mode. The fragmentation features evidenced from 36 saponin reference standards, high-accuracy MS and Fast-DDA-MS2 data, elemental composition (C < 80, H < 120, O < 50), double-bond equivalent (DBE 5-15), and searching an in-house library of Panax notoginseng, were simultaneously utilized for structural elucidation. Ultimately, 148 saponins were separated and characterized, and 80 have not been isolated from P. notoginseng. An in-depth depiction of the chemical composition of XSTI was achieved. The results obtained would benefit better understanding of the therapeutic basis and significant promotion on the quality standard of XSTI as well as other homologous products. (C) 2016 Published by Elsevier B.V. |
| WOS关键词 | CHINESE PATENT MEDICINES ; CARTHAMUS-TINCTORIUS L. ; PANAX-NOTOGINSENG ; TRITERPENOID SAPONINS ; COLUMN CHROMATOGRAPHY ; EFFICIENT DISCOVERY ; NATURAL COMPOUNDS ; IDENTIFICATION ; GINSENOSIDES ; GINSENG |
| 资助项目 | 56th China Post-doctoral Science Foundation[2014M560364] ; National Natural Science Foundation of China[81473344] ; National Natural Science Foundation of China[81503240] ; National Science and Technology Major Project for Major Drug Development[2014ZX09304-307-001-007] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000381591900039 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275898]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Wu, Wanying |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, 501 Haike Rd, Shanghai 201203, Peoples R China; 2.Guangxi Wuzhou Pharmaceut Grp Co Ltd, Wuzhou 543000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Wenzhi,Zhang, Jingxian,Yao, Changliang,et al. Method development and application of offline two-dimensional liquid chromatography/quadrupole time-of-flight mass spectrometry-fast data directed analysis for comprehensive characterization of the saponins from Xueshuantong Injection[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2016,128:322-332. |
| APA | Yang, Wenzhi.,Zhang, Jingxian.,Yao, Changliang.,Qiu, Shi.,Chen, Ming.,...&Guo, Dean.(2016).Method development and application of offline two-dimensional liquid chromatography/quadrupole time-of-flight mass spectrometry-fast data directed analysis for comprehensive characterization of the saponins from Xueshuantong Injection.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,128,322-332. |
| MLA | Yang, Wenzhi,et al."Method development and application of offline two-dimensional liquid chromatography/quadrupole time-of-flight mass spectrometry-fast data directed analysis for comprehensive characterization of the saponins from Xueshuantong Injection".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 128(2016):322-332. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。