Comprehensive Evaluation of the Metabolism of Genipin-1-beta-D-gentiobioside in Vitro and in Vivo by Using HPLC-Q-TOF
文献类型：期刊论文
| 作者 | Jiang, Pei2; Ma, Yuanjie1,3; Gao, Yu1; Li, Zhixiong1 ; Lian, Shan1,3; Xu, Zhou1; Jiang, Weixin3; Tian, Xiaoting1; Huang, Chenggang1
|
| 刊名 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
 |
| 出版日期 | 2016-07-13 |
| 卷号 | 64期号:27页码:5490-5498 |
| ISSN号 | 0021-8561 |
| 关键词 | genipin-1-beta-D-gentiobioside metabolite in vitro in vivo HPLC-Q-TOF |
| DOI | 10.1021/acs.jafc.6b01835 |
| 文献子类 | Article |
| 英文摘要 | To examine the metabolism of genipin-1-beta-D-gentiobioside (GG), its distribution and biotransformation in vivo and in vitro were investigated. Urine, plasma, feces, and various organs were collected after oral administration of GG to normal rats and pseudo-germ-free rats to evaluate GG metabolism in vivo. GG was incubated with intestinal flora and primary hepatocytes in vitro to investigate microbial and hepatic metabolism. Using HPLC-Q-TOF-LC/MS, 11 metabolites of GG were absolutely or tentatively identified in terms possible elemental compositions, retention times, and characteristics of fragmentation patterns corresponding to eight biotransformations: deglycosylation, hydroxylation, sulfate conjugation, glucuronidation, hydrogenation, demethylation, glycosylation, and dehydration. Fewer metabolites were detected in pseudo-germ-free rats than in conventional rats. Moreover, geniposide and genipin were generated by the deglycoslation of intestinal bacteria. Geniposidic acid was detected in rat primary-hepatocyte incubation. This study first explores the metabolism of GG in vivo and in vitro. The results can aid the elucidation of PK profiles and clinical usage of gardenia fruit. |
| WOS关键词 | FLIGHT MASS-SPECTROMETRY ; ZI-CHI DECOCTION ; DA-HUANG DECOCTION ; RAT PLASMA ; IRIDOID GLYCOSIDES ; IDENTIFICATION ; GENIPOSIDE ; PHARMACOKINETICS ; CONSTITUENTS ; BIOANALYSIS |
| 资助项目 | State Key Program of the National Natural Science Foundation of China[90209042] ; Key Project of Shanghai Committee of Science and Technology[13401900303] |
| WOS研究方向 | Agriculture ; Chemistry ; Food Science & Technology |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000379794300008 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275961]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Tian, Xiaoting; Huang, Chenggang |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Media, Shanghai 201203, Peoples R China 2.Harbin Univ Commerce, Ctr Res & Dev Life Sci & Environm Sci, Harbin 150076, Peoples R China; 3.Harbin Univ Commerce, Sch Pharm, Harbin 150076, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Jiang, Pei,Ma, Yuanjie,Gao, Yu,et al. Comprehensive Evaluation of the Metabolism of Genipin-1-beta-D-gentiobioside in Vitro and in Vivo by Using HPLC-Q-TOF[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2016,64(27):5490-5498. |
| APA | Jiang, Pei.,Ma, Yuanjie.,Gao, Yu.,Li, Zhixiong.,Lian, Shan.,...&Huang, Chenggang.(2016).Comprehensive Evaluation of the Metabolism of Genipin-1-beta-D-gentiobioside in Vitro and in Vivo by Using HPLC-Q-TOF.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,64(27),5490-5498. |
| MLA | Jiang, Pei,et al."Comprehensive Evaluation of the Metabolism of Genipin-1-beta-D-gentiobioside in Vitro and in Vivo by Using HPLC-Q-TOF".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 64.27(2016):5490-5498. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。