Venenum Bufonis induces rat neuroinflammation by activiating NF-kappa B pathway and attenuation of BDNF
文献类型：期刊论文
| 作者 | Bi, Qi-rui1,2; Hou, Jin-jun2 ; Qi, Peng2; Ma, Chun-hua1; Shen, Yao2; Feng, Rui-hong2; Yan, Bing-peng1,2; Wang, Jian-wei1,2; Shi, Xiao-jian1,2; Zheng, Yuan-yuan2
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| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
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| 出版日期 | 2016-06-20 |
| 卷号 | 186页码:103-110 |
| 关键词 | Brain-derived neurotrophic factor (BDNF) Inflammation Neurotoxicity NF NF- < kappa > B pathway Venenum Bufonis |
| ISSN号 | 0378-8741 |
| DOI | 10.1016/j.jep.2016.03.049 |
| 文献子类 | Article |
| 英文摘要 | Ethnopharmacological relevance: Venenum Bufonis (VB), also called toad venom, has been widely used in clinic as a cardiotonic, anohyne and antineoplastic agents both in China and other Asian countries. However, its neurotoxicity and cardiotoxicity limit its wide clinical application. Compared with extensive attention attracted with cardiotoxicity, the toxic effect of VB on Central Nervous System (CNS) is much less studied. Aim of the research: This study was performed to examine the neurotoxicity caused by VB on Sprague Dawley (SD) rats, then to clarify the mechanism in vivo by investigating its action on the neuroinflammation which possibly attributed to the activation of nuclear factor-kappa B (NF-kappa B) pathway and the attenuation of brain-derived neurotrophic factor (BDNF). Materials and methods: Rats administrated with 0.5% carboxymethyl cellulose sodium salt (CMC-Na) aqueous solution and VB (100 mg/kg, 200 mg/kg and 400 mg/kg) were sacrificed at 2 h, 4 h, 6 h, 8 h, 24 h and 48 h. The brain level of neurotransmitters and their corresponding receptors, pro-inflammatory cytokines, BDNF/TrkB and NF-kappa B pathway-related proteins were examined, respectively. Results: VB administration induced severe neurologic damage and neuroinflammation, as indicated by the disordered 5-hydroxytryptamine (5-HT), dopamine (DA) and their corresponding receptors, together with the over production of inflammatory cytokines including interleukin-6 (IL-6), interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha). VB also notably promoted the expression of p-NF-kappa Bp65, p-I kappa B alpha, p-IKK alpha and p-IKK beta and down-regulated the expression of BDNF and TrkB. Conclusion: This study demonstrates that VB triggers neurotoxicity which probably is induced by neuroinflammation via activating of NF-kappa B pathway and attenuating the expression of BDNF. (C) 2016 Elsevier Ireland Ltd. All rights reserved. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; HIPPOCAMPAL-NEURONS ; CHINESE-MEDICINE ; NEUROTROPHIC FACTOR ; MASS-SPECTROMETRY ; CORTICAL-NEURONS ; 5 BUFADIENOLIDES ; NERVOUS-SYSTEM ; TOAD VENOM ; BRAIN |
| 资助项目 | National Science and Technology Major Project for Major Drug Development[2013ZX09508104] ; National Science and Technology Major Project for Major Drug Development[2014ZX09304-307-001-007] ; Traditional Chinese Medicine Industry Research Special Project[201307002] ; Standardization of Traditional Chinese Medicines/Indigenous drugs - Chinese Academy of Sciences, China[KSZD-EW-Z-004-01] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000377832000011 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275993]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Wu, Wan-ying; Guo, Dean |
| 作者单位 | 1.China Pharmaceut Univ, Coll Tradit Chinese Med, Nanjing 210009, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Haike Rd 501, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bi, Qi-rui,Hou, Jin-jun,Qi, Peng,et al. Venenum Bufonis induces rat neuroinflammation by activiating NF-kappa B pathway and attenuation of BDNF[J]. JOURNAL OF ETHNOPHARMACOLOGY,2016,186:103-110. |
| APA | Bi, Qi-rui.,Hou, Jin-jun.,Qi, Peng.,Ma, Chun-hua.,Shen, Yao.,...&Guo, Dean.(2016).Venenum Bufonis induces rat neuroinflammation by activiating NF-kappa B pathway and attenuation of BDNF.JOURNAL OF ETHNOPHARMACOLOGY,186,103-110. |
| MLA | Bi, Qi-rui,et al."Venenum Bufonis induces rat neuroinflammation by activiating NF-kappa B pathway and attenuation of BDNF".JOURNAL OF ETHNOPHARMACOLOGY 186(2016):103-110. |
入库方式： OAI收割
来源：上海药物研究所
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