Anticonvulsant and sedative-hypnotic activity screening of pearl and nacre (mother of pearl)
文献类型：期刊论文
| 作者 | Zhang, Jing-Xian; Li, Shang-Rong; Yao, Shuai; Bi, Qi-Rui; Hou, Jin-Jun ; Cai, Lu-Ying; Han, Su-Mei; Wu, Wan-Ying; Guo, De-An
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| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
 |
| 出版日期 | 2016-04-02 |
| 卷号 | 181页码:229-235 |
| 关键词 | Pearl Nacre Anticonvulsant Sedative-hypnotic 5-HT3 GABA(B) |
| ISSN号 | 0378-8741 |
| DOI | 10.1016/j.jep.2016.01.039 |
| 文献子类 | Article |
| 英文摘要 | Ethnopharmacological relevance: Pearl and nacre are valuable traditional medicines to treat palpitations, convulsions or epilepsy in China for thousands of years. However, the active ingredients are not clear till now. Aim of the study: The main purpose of the current investigation was to assess the anticonvulsant and sedative-hypnotic activity of pearl powder and nacre powder, including their corresponding 6 protein extracts. Material and methods: Determination of the amino acid composition of the obtained protein was carried out by ultra-performance liquid chromatography (UPLC) combined with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) pre-column derivatisation. The influence of the tested drugs on loco motor activity and convulsions latency was recorded. The contents of 5-Hydroxytryptamine (5-HT) and gamma-aminobutyric acid (GABA) in brain were detected by enzyme-linked immunesorbent assay (ELISA) kits. In addition, immunohistochemistry was carried out to evaluate the changes of 5-HT3 and GABA(B). In parallel, the expressions of them were demonstrated by western blot. Results: The obtained data suggested that pearl original powder (1.1 g/kg), pearl water-soluble protein (0.2 g/kg), pearl acid-soluble protein (0.275 g/kg), pearl conchiolin protein (1.1 g/kg), nacre original powder (1.1 g/kg), nacre water-soluble protein (0.2 g/kg), nacre acid-soluble protein (0.7 g/kg) and nacre conchiolin protein (1.1 g/kg) could down-regulate the expression of 5-HT3 and up-regulate the level of GABAB to varying degrees compared with the control group. Besides, drug administration also reduced the locomotor activity and increased convulsions latency with a certain mortality. Conclusions: These findings correlated with the traditional use of pearl and nacre as sedation and tranquilization agents, thus making them interesting sources for further drug development and also providing critical important evidence for the selection of quality control markers. (C) 2016 Elsevier Ireland Ltd. All rights reserved. |
| WOS关键词 | TEMPORAL-LOBE EPILEPSY ; SEROTONIN RECEPTORS ; CORTICAL-NEURONS ; 5-HT RECEPTORS ; ACTIVATION ; PHARMACOLOGY ; HIPPOCAMPAL ; POWDER |
| 资助项目 | National High Technology Research and Development Program of China[2013AA093002] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000372761500019 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276081]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Wu, Wan-Ying |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, 501 Haike Rd, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jing-Xian,Li, Shang-Rong,Yao, Shuai,et al. Anticonvulsant and sedative-hypnotic activity screening of pearl and nacre (mother of pearl)[J]. JOURNAL OF ETHNOPHARMACOLOGY,2016,181:229-235. |
| APA | Zhang, Jing-Xian.,Li, Shang-Rong.,Yao, Shuai.,Bi, Qi-Rui.,Hou, Jin-Jun.,...&Guo, De-An.(2016).Anticonvulsant and sedative-hypnotic activity screening of pearl and nacre (mother of pearl).JOURNAL OF ETHNOPHARMACOLOGY,181,229-235. |
| MLA | Zhang, Jing-Xian,et al."Anticonvulsant and sedative-hypnotic activity screening of pearl and nacre (mother of pearl)".JOURNAL OF ETHNOPHARMACOLOGY 181(2016):229-235. |
入库方式： OAI收割
来源：上海药物研究所
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