中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Anticonvulsant and sedative-hypnotic activity screening of pearl and nacre (mother of pearl)

文献类型:期刊论文

作者Zhang, Jing-Xian; Li, Shang-Rong; Yao, Shuai; Bi, Qi-Rui; Hou, Jin-Jun; Cai, Lu-Ying; Han, Su-Mei; Wu, Wan-Ying; Guo, De-An
刊名JOURNAL OF ETHNOPHARMACOLOGY
出版日期2016-04-02
卷号181页码:229-235
关键词Pearl Nacre Anticonvulsant Sedative-hypnotic 5-HT3 GABA(B)
ISSN号0378-8741
DOI10.1016/j.jep.2016.01.039
文献子类Article
英文摘要Ethnopharmacological relevance: Pearl and nacre are valuable traditional medicines to treat palpitations, convulsions or epilepsy in China for thousands of years. However, the active ingredients are not clear till now. Aim of the study: The main purpose of the current investigation was to assess the anticonvulsant and sedative-hypnotic activity of pearl powder and nacre powder, including their corresponding 6 protein extracts. Material and methods: Determination of the amino acid composition of the obtained protein was carried out by ultra-performance liquid chromatography (UPLC) combined with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) pre-column derivatisation. The influence of the tested drugs on loco motor activity and convulsions latency was recorded. The contents of 5-Hydroxytryptamine (5-HT) and gamma-aminobutyric acid (GABA) in brain were detected by enzyme-linked immunesorbent assay (ELISA) kits. In addition, immunohistochemistry was carried out to evaluate the changes of 5-HT3 and GABA(B). In parallel, the expressions of them were demonstrated by western blot. Results: The obtained data suggested that pearl original powder (1.1 g/kg), pearl water-soluble protein (0.2 g/kg), pearl acid-soluble protein (0.275 g/kg), pearl conchiolin protein (1.1 g/kg), nacre original powder (1.1 g/kg), nacre water-soluble protein (0.2 g/kg), nacre acid-soluble protein (0.7 g/kg) and nacre conchiolin protein (1.1 g/kg) could down-regulate the expression of 5-HT3 and up-regulate the level of GABAB to varying degrees compared with the control group. Besides, drug administration also reduced the locomotor activity and increased convulsions latency with a certain mortality. Conclusions: These findings correlated with the traditional use of pearl and nacre as sedation and tranquilization agents, thus making them interesting sources for further drug development and also providing critical important evidence for the selection of quality control markers. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
WOS关键词TEMPORAL-LOBE EPILEPSY ; SEROTONIN RECEPTORS ; CORTICAL-NEURONS ; 5-HT RECEPTORS ; ACTIVATION ; PHARMACOLOGY ; HIPPOCAMPAL ; POWDER
资助项目National High Technology Research and Development Program of China[2013AA093002]
WOS研究方向Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种英语
WOS记录号WOS:000372761500019
出版者ELSEVIER IRELAND LTD
源URL[http://119.78.100.183/handle/2S10ELR8/276081]  
专题上海中药现代化研究中心
通讯作者Wu, Wan-Ying
作者单位Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, 501 Haike Rd, Shanghai 201203, Peoples R China
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Zhang, Jing-Xian,Li, Shang-Rong,Yao, Shuai,et al. Anticonvulsant and sedative-hypnotic activity screening of pearl and nacre (mother of pearl)[J]. JOURNAL OF ETHNOPHARMACOLOGY,2016,181:229-235.
APA Zhang, Jing-Xian.,Li, Shang-Rong.,Yao, Shuai.,Bi, Qi-Rui.,Hou, Jin-Jun.,...&Guo, De-An.(2016).Anticonvulsant and sedative-hypnotic activity screening of pearl and nacre (mother of pearl).JOURNAL OF ETHNOPHARMACOLOGY,181,229-235.
MLA Zhang, Jing-Xian,et al."Anticonvulsant and sedative-hypnotic activity screening of pearl and nacre (mother of pearl)".JOURNAL OF ETHNOPHARMACOLOGY 181(2016):229-235.

入库方式: OAI收割

来源:上海药物研究所

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