Possible target-related proteins and signal network of bufalin in A549 cells suggested by both iTRAQ-based and label-free proteomic analysis
文献类型：期刊论文
| 作者 | Zhang, Dong-Mei4,5; Feng, Li-Xing5; Liu, Miao5; Jin, Wen-Hai; Luo, Ji; Nie, Ai-Ying2; Zhou, Yue2; Li, Yin1; Wu, Wan-Ying3,5 ; Jiang, Bao-Hong3,5
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| 刊名 | PROTEOMICS
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| 出版日期 | 2016-03 |
| 卷号 | 16期号:6页码:935-945 |
| 关键词 | A549 cells Bufalin Cell biology iTRAQ-based Label-free |
| ISSN号 | 1615-9853 |
| DOI | 10.1002/pmic.201500418 |
| 文献子类 | Article |
| 英文摘要 | Bufalin (BF) exhibited antiproliferation and antimigration effects on human A549 lung cancer cells. To search its target-related proteins, protein expression profiles of BF-treated and control cells were compared using two quantitative proteomic methods, iTRAQ-based and label-free proteomic analysis. A total of 5428 proteins were identified in iTRAQ-based analysis while 6632 proteins were identified in label-free analysis. The number of common identified proteins of both methods was 4799 proteins. By application of 1.20-fold for upregulated and 0.83-fold for downregulated cutoff values, 273 and 802 differentially expressed proteins were found in iTRAQ-based and label-free analysis, respectively. The number of common differentially expressed proteins of both methods was 45 proteins. Results of bioinformational analysis using Metacore(TM) showed that the two proteomic methods were complementary and both suggested the involvement of oxidative stress and regulation of gene expression in the effects of BF, and fibronectin-related pathway was suggested to be an important pathway affected by BF. Western blotting assay results confirmed BF-induced change in levels of fibronectin and other related proteins. Overexpression of fibronectin by plasmid transfection ameliorated antimigration effects of BF. Results of the present study provided information about possible target-related proteins and signal network of BF. |
| WOS关键词 | EXTRACELLULAR-MATRIX REGULATION ; LUNG-CANCER ; DRUG-RESISTANCE ; HEPATOCELLULAR-CARCINOMA ; CARDIOTONIC STEROIDS ; NA+/K+-ATPASE ; FIBRONECTIN ; APOPTOSIS ; KINASE ; INHIBITORS |
| 资助项目 | Shanghai Science & Technology Support Program[13431900401] ; Shanghai Science & Technology Innovation Action Program[15140904800] ; National Nature Science Foundation of China[81373964] ; National Science & Technology Major Project of China[2014ZX09301-306-03] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000372976400005 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276118]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Liu, Xuan |
| 作者单位 | 1.Thomson Reuters, Shanghai, Peoples R China 2.Thermo Fisher Sci, Shanghai, Peoples R China; 3.AB Sciex, Shanghai, Peoples R China; 4.Chinese PLA, Lanzhou Mil Command, Lanzhou Gen Hosp, Dept Pharm, Lanzhou, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhang, Dong-Mei,Feng, Li-Xing,Liu, Miao,et al. Possible target-related proteins and signal network of bufalin in A549 cells suggested by both iTRAQ-based and label-free proteomic analysis[J]. PROTEOMICS,2016,16(6):935-945. |
| APA | Zhang, Dong-Mei.,Feng, Li-Xing.,Liu, Miao.,Jin, Wen-Hai.,Luo, Ji.,...&Liu, Xuan.(2016).Possible target-related proteins and signal network of bufalin in A549 cells suggested by both iTRAQ-based and label-free proteomic analysis.PROTEOMICS,16(6),935-945. |
| MLA | Zhang, Dong-Mei,et al."Possible target-related proteins and signal network of bufalin in A549 cells suggested by both iTRAQ-based and label-free proteomic analysis".PROTEOMICS 16.6(2016):935-945. |
入库方式： OAI收割
来源：上海药物研究所
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