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Selective ion monitoring of quinochalcone C-glycoside markers for the simultaneous identification of Carthamus tinctorius L. in eleven Chinese patent medicines by UHPLC/QTOF MS

文献类型：期刊论文

作者Si, Wei1,2; Yang, Wenzhi2; Guo, Dean2; Wu, Jia2; Zhang, Jingxian2; Qiu, Shi2; Yao, Changliang2; Cui, Yajun1; Wu, Wanying2
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
出版日期2016-01-05
卷号117页码:510-521
关键词Selective ion monitoring UHPLC/QTOF MS Chinese patent medicine Carthamus tinctorius L. Quinochalcone C-glycoside
ISSN号0731-7085
DOI10.1016/j.jpba.2015.09.025
文献子类Article
英文摘要Current China Pharmacopoeia standards for the Chinese patent medicines (CPMs) that contain one or several the same drug(s) employ case-dependent TLC or HPLC approaches to achieve qualitative identification. A qualitative "monomethod-heterotrait matrix" (MHM) strategy is thus proposed, by selective monitoring of multi-biomarkers, to achieve the identification of different CPMs. Carthamus tinctorius L. (safflower) is a reputable gynecological herbal medicine containing characteristic quinochalcone C-glycosides (QCGs) as the major bioactive components. Qualitative identification of safflower in diverse CPMs by selective monitoring of QCG markers was performed by use of the MHM strategy. Initially, 27 QCG analogs (involving 16 potentially new ones) were selectively characterized by product ion filtering (m/z 119.05) and integrated analysis of the negative mode MSE and Fast DDA data obtained on a UHPLC/QTOF mass spectrometer. Subsequently, by fingerprint analysis of 20 batches of safflower samples followed by. a thermostable test, six QCGs (hydroxysafflor yellow A and its two isomers, anhydrosafflor yellow B, safflomin C, and isosafflomin C) were selected as the biomarkers for safflower. Then, a highly specific selective ion monitoring (SIM) method by recording centroided data was developed and applied to selectively profile six QCG biomarkers from 28 batches of CPM samples collected from versatile vendors. By reference to a standard SIM spectrum established using a home-made safflower reference extract, simultaneous identification of safflower in eleven different CPMs was accomplished with the unified sample preparation and a single UHPLC/QTOF-SIM method. The qualitative MHM strategy represents the novel methodology that facilitates the quality control of CPMs more efficiently. (C) 2015 Elsevier B.V. All rights reserved.
WOS关键词FLIGHT MASS-SPECTROMETRY ; CHROMATOGRAPHY-QUADRUPOLE-TIME ; RAPID CHARACTERIZATION ; HOLISTIC QUALITY ; PANAX-GINSENG ; COMPONENTS ; ARRAY ; QUANTIFICATION ; CONSTITUENTS ; METABOLOMICS
资助项目Twelfth Five-Year National Science & Technology Support Program[2012BAI29B06] ; National Science and Technology Major Project for Major Drug Development[2013ZX09508104] ; National Science and Technology Major Project for Major Drug Development[2014ZX09304-307-001-007] ; National High Technology Research and Development Program of China[2013AA093002]
WOS研究方向Chemistry ; Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000365145500062
出版者ELSEVIER SCIENCE BV
源URL[http://119.78.100.183/handle/2S10ELR8/276184]  
专题上海中药现代化研究中心
通讯作者Cui, Yajun
作者单位1.Shanghai Univ Tradit Chinese Med, Shanghai 201203, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China;
推荐引用方式
GB/T 7714		 Si, Wei,Yang, Wenzhi,Guo, Dean,et al. Selective ion monitoring of quinochalcone C-glycoside markers for the simultaneous identification of Carthamus tinctorius L. in eleven Chinese patent medicines by UHPLC/QTOF MS[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2016,117:510-521.
APA Si, Wei.,Yang, Wenzhi.,Guo, Dean.,Wu, Jia.,Zhang, Jingxian.,...&Wu, Wanying.(2016).Selective ion monitoring of quinochalcone C-glycoside markers for the simultaneous identification of Carthamus tinctorius L. in eleven Chinese patent medicines by UHPLC/QTOF MS.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,117,510-521.
MLA Si, Wei,et al."Selective ion monitoring of quinochalcone C-glycoside markers for the simultaneous identification of Carthamus tinctorius L. in eleven Chinese patent medicines by UHPLC/QTOF MS".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 117(2016):510-521.

入库方式： OAI收割

来源：上海药物研究所

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