A strategy for establishment of practical identification methods for Chinese patent medicine from systematic multi-component characterization to selective ion monitoring of chemical markers: Shuxiong tablet as a case study
文献类型：期刊论文
| 作者 | Yao, Changliang1,2; Yang, Wenzhi1; Si, Wei1; Pan, Huiqin1,2; Qiu, Shi1; Wu, Jia1,2; Shi, Xiaojian1; Feng, Ruihong1; Wu, Wanying1 ; Guo, Dean1
|
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2016 |
| 卷号 | 6期号:69页码:65055-65066 |
| ISSN号 | 2046-2069 |
| DOI | 10.1039/c6ra10883k |
| 文献子类 | Article |
| 英文摘要 | Authentication of Chinese patent medicine (CPM) is more challenging than that of a single herbal medicine due to the increasing complexity in the chemical matrix. A strategy from systematic multi-component characterization to selective ion monitoring of chemical markers (SMC-SIM) is presented and applied to authenticate Shuxiong tablet (SXT) as a case study. First, an ultra-performance liquid chromatography/quadrupole time-of-flight-Fast DDA (UPLC/QTOF-Fast DDA) approach was developed to comprehensively profile and characterize multi-components in SXT. Second, chemical markers were established using a home-made SXT sample. The SIM method for detection of these markers was initially established on an advanced QTOF mass spectrometer, and then transferred onto an easily accessible single quadrupole QDa detector. Third, the UPLC/QDa-SIM method was used to authenticate commercial SXT samples. Consequently, 250 compounds were characterized, and 73 of them were identified by comparison with reference standards. Two UPLC-SIM methods enabling the monitoring of eleven chemical markers (including five saponins for Notoginseng Radix et Rhizoma, two quinochalcone C-glycosides and a flavonoid O-glycoside for Carthami Flos, one phenolic acid and two phthalides for Chuanxiong Rhizoma), were developed on QTOF and QDa, respectively. Twelve batches of commercial SXT samples were identified by the UPLC/QDa-SIM method. Despite the lower sensitivity, the QDa-SIM method by simple sample preparation achieved similar authentication results, and was more easily generalized in practice than the QTOF-SIM method. In conclusion, targeted monitoring of multiple chemical markers was proven as feasible in comprehensive identification of CPM, and the SMC-SIM strategy enabled establishment of practical quality control methods for CPM that contains a complex chemical matrix. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; CARTHAMUS-TINCTORIUS L. ; PANAX-NOTOGINSENG ; COLUMN CHROMATOGRAPHY ; EFFICIENT DISCOVERY ; INTEGRATED STRATEGY ; QUALITY EVALUATION ; CHLOROGENIC ACIDS ; NATURAL COMPOUNDS |
| 资助项目 | National Natural Science Foundation of China[81503240] ; National Natural Science Foundation of China[81473344] ; National Science and Technology Major Project for Major Drug Development[2014ZX09304-307-001-007] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000379442400099 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276216]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Wu, Wanying |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, 501 Haike Rd, Shanghai 201203, Peoples R China; 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yao, Changliang,Yang, Wenzhi,Si, Wei,et al. A strategy for establishment of practical identification methods for Chinese patent medicine from systematic multi-component characterization to selective ion monitoring of chemical markers: Shuxiong tablet as a case study[J]. RSC ADVANCES,2016,6(69):65055-65066. |
| APA | Yao, Changliang.,Yang, Wenzhi.,Si, Wei.,Pan, Huiqin.,Qiu, Shi.,...&Guo, Dean.(2016).A strategy for establishment of practical identification methods for Chinese patent medicine from systematic multi-component characterization to selective ion monitoring of chemical markers: Shuxiong tablet as a case study.RSC ADVANCES,6(69),65055-65066. |
| MLA | Yao, Changliang,et al."A strategy for establishment of practical identification methods for Chinese patent medicine from systematic multi-component characterization to selective ion monitoring of chemical markers: Shuxiong tablet as a case study".RSC ADVANCES 6.69(2016):65055-65066. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。