Simultaneous determination of gypenoside LVI, gypenoside XLVI, 2 alpha-OH-protopanaxadiol and their two metabolites in rat plasma by LC MS/MS and its application to pharmacokinetic studies
文献类型：期刊论文
| 作者 | Zhang, Lihua2; Lin, Yunfei1; Guan, Huashi2; Hu, Lihong1; Pan, Guoyu1
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2015-11-15 |
| 卷号 | 1005页码:9-16 |
| 关键词 | Gypenoside 2 alpha-OH-Protopanaxadiol Gynostemma pentaphyllum Validation Pharmacokinetic |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2015.09.028 |
| 文献子类 | Article |
| 英文摘要 | Gypenoside LVI and gypenoside XLVI are the major bioactive dammarane saponins from Gynostemma pentaphyllum. Gypenoside LVI, gypenoside XLVI, and their metabolite 2 alpha-OH-protopanaxadiol (2 alpha-OH-PPD) possess potent non-small cell lung carcinoma A549 cell inhibitory activity. A sensitive liquid chromatography tandem mass spectrometry method was developed and validated to study the pharmacokinetics of gypenoside LVI and XLVI, 2 alpha-OH-PPD, metabolite 1 (M1), and metabolite 2 (M2) after administration of gypenosides or 2 alpha-OH-PPD. Plasma samples from rats were protein precipitated with methanol. Analytes were detected by triple quadrupole MS/MS with an electrospray ionization source in the positive multiple reaction monitoring mode. The transition m/z 441.4 -> 109.2 was selected to quantify gypenoside LVI and XLVI, and 2 alpha-OH-PPD, because of the extensive conversion of the gypenosides to aglycone in the ionization source. M1 and M2 are isomers that shared the transition m/z 493.4 -> 143.1. To avoid interference, the baseline separation of each analyte was performed on a SunFire C18 column with a gradient of acetonitrile (0.1% formic acid, v/v) and water (0.1% formic acid, v/v). The chromatographic run time was 10 min. The linearity was validated over a plasma concentration range from 2.00 to 2000 ng/mL for M1 and M2, and from 10.0 to 2000 for gypenosides LVI and XLVI, and 2 alpha-OH-protopanaxadiol. The lower limits of quantification were 10.0, 10.0, 10.0, 2.00, and 2.00 ng/mL for gypenoside LVI, gypenoside XLVI, 2 alpha-OH-PPD,M1, and M2, respectively, with acceptable intra-/inter-day precision and accuracy. The extraction recovery rates were >86.9% for each compound. No apparent matrix effect or instability was observed during each step of the bioanalysis. After full validation, this method was proved to be simple, fast, and efficient in analyzing large batches of plasma samples for the analytes. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; GYNOSTEMMA-PENTAPHYLLUM ; CELLS ; QUANTIFICATION ; MODEL |
| 资助项目 | National High Technology Research and Development Program of China[2013AA032202] ; National Natural Science Foundation of China[81302836] ; National Natural Science Foundation of China[81573499] ; National Natural Science Foundation of China[1009936] ; Special Project of Science and Technology of the Chinese Academy of Sciences[XDA01030600] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000364727000002 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276322]  |
| 专题 | 药物安全性评价中心 上海中药现代化研究中心 |
| 通讯作者 | Hu, Lihong |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 2.Ocean Univ China, Sch Med & Pharm, Qingdao 266003, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhang, Lihua,Lin, Yunfei,Guan, Huashi,et al. Simultaneous determination of gypenoside LVI, gypenoside XLVI, 2 alpha-OH-protopanaxadiol and their two metabolites in rat plasma by LC MS/MS and its application to pharmacokinetic studies[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2015,1005:9-16. |
| APA | Zhang, Lihua,Lin, Yunfei,Guan, Huashi,Hu, Lihong,&Pan, Guoyu.(2015).Simultaneous determination of gypenoside LVI, gypenoside XLVI, 2 alpha-OH-protopanaxadiol and their two metabolites in rat plasma by LC MS/MS and its application to pharmacokinetic studies.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1005,9-16. |
| MLA | Zhang, Lihua,et al."Simultaneous determination of gypenoside LVI, gypenoside XLVI, 2 alpha-OH-protopanaxadiol and their two metabolites in rat plasma by LC MS/MS and its application to pharmacokinetic studies".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1005(2015):9-16. |
入库方式： OAI收割
来源：上海药物研究所
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