A green protocol for efficient discovery of novel natural compounds: Characterization of new ginsenosides from the stems and leaves of Panax ginseng as a case study
文献类型：期刊论文
| 作者 | Qiu, Shi; Yang, Wen-zhi; Shi, Xiao-jian; Yao, Chang-liang; Yang, Min; Liu, Xuan; Jiang, Bao-hong ; Wu, Wan-ying; Guo, De-an
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| 刊名 | ANALYTICA CHIMICA ACTA
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| 出版日期 | 2015-09-17 |
| 卷号 | 893页码:65-76 |
| 关键词 | Offline comprehensive 2D LC LTQ-Orbitrap-MS Stems and leaves of Panax ginseng Ginsenoside HCD Sapogenin diversity |
| ISSN号 | 0003-2670 |
| DOI | 10.1016/j.aca.2015.08.048 |
| 文献子类 | Article |
| 英文摘要 | Exploration of new natural compounds is of vital significance for drug discovery and development. The conventional approaches by systematic phytochemical isolation are low-efficiency and consume masses of organic solvent. This study presents an integrated strategy that combines offline comprehensive two-dimensional liquid chromatography, hybrid linear ion-trap/Orbitrap mass spectrometry, and NMR analysis (2D LC/LTQ-Orbitrap-MS/NMR), aimed to establish a green protocol for the efficient discovery of new natural molecules. A comprehensive chemical analysis of the total ginsenosides of stems and leaves of Panax ginseng (SLP), a cardiovascular disease medicine, was performed following this strategy. An offline 2D LC system was constructed with an orthogonality of 0.79 and a practical peak capacity of 11,000. The much greener UHPLC separation and LTQ-Orbitrap-MS detection by data-dependent highenergy C-trap dissociation (HCD)/dynamic exclusion were employed for separation and characterization of ginsenosides from thirteen fractionated SLP samples. Consequently, a total of 646 ginsenosides were characterized, and 427 have not been isolated from the genus of Panax L. The ginsenosides identified from SLP exhibited distinct sapogenin diversity and molecular isomerism. NMR analysis was finally employed to verify and offer complementary structural information to MS-oriented characterization. The established 2D LC/LTQ-Orbitrap-MS/NMR approach outperforms the conventional approaches in respect of significantly improved efficiency, much less use of drug materials and organic solvent. The integrated strategy enables a deep investigation on the therapeutic basis of an herbal medicine, and facilitates new compounds discovery in an efficient and environmentally friendly manner as well. (C) 2015 Elsevier B.V. All rights reserved. |
| WOS关键词 | 2-DIMENSIONAL LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; LTQ-ORBITRAP ; COLUMN CHROMATOGRAPHY ; FLOWER BUDS ; SAPONINS ; IDENTIFICATION ; NOTOGINSENG ; SEPARATION ; CONSTITUENTS |
| 资助项目 | National Science and Technology Major Project for Major Drug Development[2013ZX09508104] ; National Science and Technology Major Project for Major Drug Development[2014ZX09304-307-001-007] ; Twelfth Five-Year National Science & Technology Support Program[2012BAI29B06] ; Special Scientific Research in the Chinese Medicine Industry of State Administration of Traditional Chinese Medicine[201307002] ; National Natural Science Foundation of China[81473344] ; National Natural Science Foundation of China[81503240] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000361647200008 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276395]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Wu, Wan-ying |
| 作者单位 | Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiu, Shi,Yang, Wen-zhi,Shi, Xiao-jian,et al. A green protocol for efficient discovery of novel natural compounds: Characterization of new ginsenosides from the stems and leaves of Panax ginseng as a case study[J]. ANALYTICA CHIMICA ACTA,2015,893:65-76. |
| APA | Qiu, Shi.,Yang, Wen-zhi.,Shi, Xiao-jian.,Yao, Chang-liang.,Yang, Min.,...&Guo, De-an.(2015).A green protocol for efficient discovery of novel natural compounds: Characterization of new ginsenosides from the stems and leaves of Panax ginseng as a case study.ANALYTICA CHIMICA ACTA,893,65-76. |
| MLA | Qiu, Shi,et al."A green protocol for efficient discovery of novel natural compounds: Characterization of new ginsenosides from the stems and leaves of Panax ginseng as a case study".ANALYTICA CHIMICA ACTA 893(2015):65-76. |
入库方式： OAI收割
来源：上海药物研究所
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