中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
In-vivo and In-vitro Metabolism Study of Timosaponin B-II Using HPLC-ESI-MS (n)

文献类型:期刊论文

作者Jia, Yao2,3; Fu, Zhiwen1,2; Li, Zhixiong2; Hu, Pei2; Xue, Rui2; Chen, Mingcang2; Xiang, Ting3; Huang, Chenggang2
刊名CHROMATOGRAPHIA
出版日期2015-09
卷号78期号:17-18页码:1175-1184
关键词HPLC-ESI-MSn Timosaponin B-II Metabolism Tissue distribution
ISSN号0009-5893
DOI10.1007/s10337-015-2927-6
文献子类Article
英文摘要Timosaponin B-II (TB-II), a representative furostanol saponin in Rhizoma anemarrhenae, has been used as an emperor herb in many Chinese herbal formulas to treat diabetes and senile dementia. However, its metabolism and tissue distribution had not been investigated so far. In this work, a sensitive and specific high-performance liquid chromatography-electrospray ionization tandem mass spectrometry method was applied for the identification of TB-II and its major metabolites in in-vivo and in-vitro samples. Rat urine, feces, plasma and tissues were collected after oral administration of TB-II at a single dose of 300 mg kg(-1). Furthermore, TB-II was incubated in artificial gastric juice (AGJ) and artificial intestinal juice (AIJ). As a result, 19 metabolites were detected and identified by comparing their HPLC behavior and MSn spectra profile with those of the parent drug. Moreover, the structures of its five metabolites were identified by using the standards prepared by the acid hydrolysis of TB-II. In addition to the parent drug, 14, 12, 6, 1, 1 and 7 metabolites were detected in rat urine, feces, plasma, heart, kidney and liver, respectively, while no metabolites or the parent drug were found in rat brain, spleen and lung. Seven metabolites appeared in AIJ incubation samples, but the parent drug was absent. Nine metabolites along with the parent drug were observed in AGJ incubation samples. The biotransformation pathways of TB-II mainly included dehydration, deglycosylation, hydroxylation, oxidation and E-ring cleavage. This is the first comprehensive investigation of the in-vivo and in-vitro metabolism of TB-II. The result provided important information for further pharmacological research on TB-II.
WOS关键词FLIGHT MASS-SPECTROMETRY ; ANEMARRHENA-ASPHODELOIDES ; LIQUID-CHROMATOGRAPHY ; STEROIDAL SAPONINS ; RAT PLASMA ; HERB-PAIR ; A-III ; EXTRACT ; URINE ; IDENTIFICATION
资助项目Key Program and General Program of National Natural Science Foundation of China[81030065] ; Key Program and General Program of National Natural Science Foundation of China[81274055]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000360288100008
出版者SPRINGER HEIDELBERG
源URL[http://119.78.100.183/handle/2S10ELR8/276423]  
专题上海中药现代化研究中心
通讯作者Xiang, Ting
作者单位1.Shanghai Univ Tradit Chinese Med, Shanghai, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
3.Yangtze River Pharmaceut Grp, Pharmaceut Res Inst, Beijing 102206, Peoples R China;
推荐引用方式
GB/T 7714
Jia, Yao,Fu, Zhiwen,Li, Zhixiong,et al. In-vivo and In-vitro Metabolism Study of Timosaponin B-II Using HPLC-ESI-MS (n)[J]. CHROMATOGRAPHIA,2015,78(17-18):1175-1184.
APA Jia, Yao.,Fu, Zhiwen.,Li, Zhixiong.,Hu, Pei.,Xue, Rui.,...&Huang, Chenggang.(2015).In-vivo and In-vitro Metabolism Study of Timosaponin B-II Using HPLC-ESI-MS (n).CHROMATOGRAPHIA,78(17-18),1175-1184.
MLA Jia, Yao,et al."In-vivo and In-vitro Metabolism Study of Timosaponin B-II Using HPLC-ESI-MS (n)".CHROMATOGRAPHIA 78.17-18(2015):1175-1184.

入库方式: OAI收割

来源:上海药物研究所

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