In-vivo and In-vitro Metabolism Study of Timosaponin B-II Using HPLC-ESI-MS (n)
文献类型：期刊论文
| 作者 | Jia, Yao2,3; Fu, Zhiwen1,2; Li, Zhixiong2 ; Hu, Pei2; Xue, Rui2; Chen, Mingcang2; Xiang, Ting3; Huang, Chenggang2
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| 刊名 | CHROMATOGRAPHIA
 |
| 出版日期 | 2015-09 |
| 卷号 | 78期号:17-18页码:1175-1184 |
| 关键词 | HPLC-ESI-MSn Timosaponin B-II Metabolism Tissue distribution |
| ISSN号 | 0009-5893 |
| DOI | 10.1007/s10337-015-2927-6 |
| 文献子类 | Article |
| 英文摘要 | Timosaponin B-II (TB-II), a representative furostanol saponin in Rhizoma anemarrhenae, has been used as an emperor herb in many Chinese herbal formulas to treat diabetes and senile dementia. However, its metabolism and tissue distribution had not been investigated so far. In this work, a sensitive and specific high-performance liquid chromatography-electrospray ionization tandem mass spectrometry method was applied for the identification of TB-II and its major metabolites in in-vivo and in-vitro samples. Rat urine, feces, plasma and tissues were collected after oral administration of TB-II at a single dose of 300 mg kg(-1). Furthermore, TB-II was incubated in artificial gastric juice (AGJ) and artificial intestinal juice (AIJ). As a result, 19 metabolites were detected and identified by comparing their HPLC behavior and MSn spectra profile with those of the parent drug. Moreover, the structures of its five metabolites were identified by using the standards prepared by the acid hydrolysis of TB-II. In addition to the parent drug, 14, 12, 6, 1, 1 and 7 metabolites were detected in rat urine, feces, plasma, heart, kidney and liver, respectively, while no metabolites or the parent drug were found in rat brain, spleen and lung. Seven metabolites appeared in AIJ incubation samples, but the parent drug was absent. Nine metabolites along with the parent drug were observed in AGJ incubation samples. The biotransformation pathways of TB-II mainly included dehydration, deglycosylation, hydroxylation, oxidation and E-ring cleavage. This is the first comprehensive investigation of the in-vivo and in-vitro metabolism of TB-II. The result provided important information for further pharmacological research on TB-II. |
| WOS关键词 | FLIGHT MASS-SPECTROMETRY ; ANEMARRHENA-ASPHODELOIDES ; LIQUID-CHROMATOGRAPHY ; STEROIDAL SAPONINS ; RAT PLASMA ; HERB-PAIR ; A-III ; EXTRACT ; URINE ; IDENTIFICATION |
| 资助项目 | Key Program and General Program of National Natural Science Foundation of China[81030065] ; Key Program and General Program of National Natural Science Foundation of China[81274055] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000360288100008 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276423]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Xiang, Ting |
| 作者单位 | 1.Shanghai Univ Tradit Chinese Med, Shanghai, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.Yangtze River Pharmaceut Grp, Pharmaceut Res Inst, Beijing 102206, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Jia, Yao,Fu, Zhiwen,Li, Zhixiong,et al. In-vivo and In-vitro Metabolism Study of Timosaponin B-II Using HPLC-ESI-MS (n)[J]. CHROMATOGRAPHIA,2015,78(17-18):1175-1184. |
| APA | Jia, Yao.,Fu, Zhiwen.,Li, Zhixiong.,Hu, Pei.,Xue, Rui.,...&Huang, Chenggang.(2015).In-vivo and In-vitro Metabolism Study of Timosaponin B-II Using HPLC-ESI-MS (n).CHROMATOGRAPHIA,78(17-18),1175-1184. |
| MLA | Jia, Yao,et al."In-vivo and In-vitro Metabolism Study of Timosaponin B-II Using HPLC-ESI-MS (n)".CHROMATOGRAPHIA 78.17-18(2015):1175-1184. |
入库方式： OAI收割
来源：上海药物研究所
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