中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Simultaneous determination and assignment of 13 major flavonoids and glycyrrhizic acid in licorices by HPLC-DAD and Orbirap mass spectrometry analyses

文献类型:期刊论文

作者Wei Shan-Shan1; Yang Min2; Chen Xin2; Wang Qiu-Rong2; Cui Ya-Jun1
刊名CHINESE JOURNAL OF NATURAL MEDICINES
出版日期2015-03
卷号13期号:3页码:232-240
关键词Licorice Flavonoids Glycyrrhizic acid Simultaneous determination HPLC-DAD Orbitrap mass spectrometry
ISSN号2095-6975
DOI10.3724/SP.J.1009.2015.00232
文献子类Article
英文摘要To determine 13 flavonoids and glycyrrhizic acid in licorice (Glycyrrhiza spp.), several samples from different areas were examined by HPLC-DAD analysisThe analysis was performed on a Zorbax Extend-C-18 (250 mm x 4.6 mm, 5 mu m) column connected with a Zorbax Extend guard column (20 mm x 4.6 mm, 5 mu m). The mobile phase consisted of (A) acetonitrile and (B) 0.026% aqueous H3PO4 (V/V) using a gradient elution of 20%-25% A at 0-20 min, 25%-33% A at 20-30 min, 33%-50% A at 30-55 min, 50%-60% A at 55-65 min, and 60% A between 65 min and 80 min, and peaks were detected at 280 urn. The fourteen compounds were assigned by HPLC-Orbitrap MS methods. The regression coefficient for the linear equations for the 14 compounds ranged between 0.9998 and 1. The limits of detection and quantification lay in the range of 0.032-2.461 and 0.154-8.202 mu g.mL(-1), respectively. The relative recovery rates for the 14 compounds were in the range of 93.90%-106.73% with RSDs being less than 5%. Coefficient variations for intra-day and inter-day precisions were in the range of 0.27%-2.38% and 0.31%-3.51%, respectively. In summary, the validated method was applied to the simultaneous determination of the 14 components in 29 different licorice samples and was proven to be suitable for quality evaluation of licorices and their active fractions.
WOS关键词CANCER CELLS ; URALENSIS ; LIQUIRITIN ; CHROMATOGRAPHY ; CONSTITUENTS ; EXTRACT ; GLABRA ; ROOT ; ISOLIQUIRITIGENIN ; PAEONIFLORIN
资助项目National Natural Science Foundation of China[81001630] ; Natural Science Foundation of Shanghai[10ZR1436400] ; Major Projects of Knowledge Innovation Program of the Chinese Academy of Sciences[KSCX2-YW-R-166] ; Twelfth Five-Year National Science & Technology Support Program[2012BAI29B06]
WOS研究方向Integrative & Complementary Medicine ; Pharmacology & Pharmacy
语种英语
CSCD记录号CSCD:5380446
WOS记录号WOS:000355067700009
出版者CHINESE JOURNAL NATURAL MEDICINES
源URL[http://119.78.100.183/handle/2S10ELR8/276615]  
专题上海中药现代化研究中心
通讯作者Cui Ya-Jun
作者单位1.Shanghai Univ Tradit Chinese Med, Sch Chinese Mat Med, Shanghai 201203, Peoples R China;
2.Chinese Acad Sci, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr TCM Modernizat, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714
Wei Shan-Shan,Yang Min,Chen Xin,et al. Simultaneous determination and assignment of 13 major flavonoids and glycyrrhizic acid in licorices by HPLC-DAD and Orbirap mass spectrometry analyses[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2015,13(3):232-240.
APA Wei Shan-Shan,Yang Min,Chen Xin,Wang Qiu-Rong,&Cui Ya-Jun.(2015).Simultaneous determination and assignment of 13 major flavonoids and glycyrrhizic acid in licorices by HPLC-DAD and Orbirap mass spectrometry analyses.CHINESE JOURNAL OF NATURAL MEDICINES,13(3),232-240.
MLA Wei Shan-Shan,et al."Simultaneous determination and assignment of 13 major flavonoids and glycyrrhizic acid in licorices by HPLC-DAD and Orbirap mass spectrometry analyses".CHINESE JOURNAL OF NATURAL MEDICINES 13.3(2015):232-240.

入库方式: OAI收割

来源:上海药物研究所

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