Simultaneous determination and assignment of 13 major flavonoids and glycyrrhizic acid in licorices by HPLC-DAD and Orbirap mass spectrometry analyses
文献类型：期刊论文
| 作者 | Wei Shan-Shan1; Yang Min2; Chen Xin2; Wang Qiu-Rong2; Cui Ya-Jun1 |
| 刊名 | CHINESE JOURNAL OF NATURAL MEDICINES
 |
| 出版日期 | 2015-03 |
| 卷号 | 13期号:3页码:232-240 |
| 关键词 | Licorice Flavonoids Glycyrrhizic acid Simultaneous determination HPLC-DAD Orbitrap mass spectrometry |
| ISSN号 | 2095-6975 |
| DOI | 10.3724/SP.J.1009.2015.00232 |
| 文献子类 | Article |
| 英文摘要 | To determine 13 flavonoids and glycyrrhizic acid in licorice (Glycyrrhiza spp.), several samples from different areas were examined by HPLC-DAD analysisThe analysis was performed on a Zorbax Extend-C-18 (250 mm x 4.6 mm, 5 mu m) column connected with a Zorbax Extend guard column (20 mm x 4.6 mm, 5 mu m). The mobile phase consisted of (A) acetonitrile and (B) 0.026% aqueous H3PO4 (V/V) using a gradient elution of 20%-25% A at 0-20 min, 25%-33% A at 20-30 min, 33%-50% A at 30-55 min, 50%-60% A at 55-65 min, and 60% A between 65 min and 80 min, and peaks were detected at 280 urn. The fourteen compounds were assigned by HPLC-Orbitrap MS methods. The regression coefficient for the linear equations for the 14 compounds ranged between 0.9998 and 1. The limits of detection and quantification lay in the range of 0.032-2.461 and 0.154-8.202 mu g.mL(-1), respectively. The relative recovery rates for the 14 compounds were in the range of 93.90%-106.73% with RSDs being less than 5%. Coefficient variations for intra-day and inter-day precisions were in the range of 0.27%-2.38% and 0.31%-3.51%, respectively. In summary, the validated method was applied to the simultaneous determination of the 14 components in 29 different licorice samples and was proven to be suitable for quality evaluation of licorices and their active fractions. |
| WOS关键词 | CANCER CELLS ; URALENSIS ; LIQUIRITIN ; CHROMATOGRAPHY ; CONSTITUENTS ; EXTRACT ; GLABRA ; ROOT ; ISOLIQUIRITIGENIN ; PAEONIFLORIN |
| 资助项目 | National Natural Science Foundation of China[81001630] ; Natural Science Foundation of Shanghai[10ZR1436400] ; Major Projects of Knowledge Innovation Program of the Chinese Academy of Sciences[KSCX2-YW-R-166] ; Twelfth Five-Year National Science & Technology Support Program[2012BAI29B06] |
| WOS研究方向 | Integrative & Complementary Medicine ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5380446 |
| WOS记录号 | WOS:000355067700009 |
| 出版者 | CHINESE JOURNAL NATURAL MEDICINES |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276615]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Cui Ya-Jun |
| 作者单位 | 1.Shanghai Univ Tradit Chinese Med, Sch Chinese Mat Med, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr TCM Modernizat, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei Shan-Shan,Yang Min,Chen Xin,et al. Simultaneous determination and assignment of 13 major flavonoids and glycyrrhizic acid in licorices by HPLC-DAD and Orbirap mass spectrometry analyses[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2015,13(3):232-240. |
| APA | Wei Shan-Shan,Yang Min,Chen Xin,Wang Qiu-Rong,&Cui Ya-Jun.(2015).Simultaneous determination and assignment of 13 major flavonoids and glycyrrhizic acid in licorices by HPLC-DAD and Orbirap mass spectrometry analyses.CHINESE JOURNAL OF NATURAL MEDICINES,13(3),232-240. |
| MLA | Wei Shan-Shan,et al."Simultaneous determination and assignment of 13 major flavonoids and glycyrrhizic acid in licorices by HPLC-DAD and Orbirap mass spectrometry analyses".CHINESE JOURNAL OF NATURAL MEDICINES 13.3(2015):232-240. |
入库方式： OAI收割
来源：上海药物研究所
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