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Characterization and profiling of phenolic amides from Cortex Lycii by ultra-high performance liquid chromatography coupled with LTQ-Orbitrap mass spectrometry

文献类型：期刊论文

作者Zhang, Jingxian1; Guan, Shuhong1; Sun, Jianghao2; Liu, Tian3; Chen, Pei2; Feng, Ruihong1; Chen, Xin1; Wu, Wanying1; Yang, Min1; Guo, De-an1
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
出版日期2015-01
卷号407期号:2页码:581-595
关键词Phenolic amide Cortex Lycii UHPLC/LTQ-Orbitrap Structural characterization
ISSN号1618-2642
DOI10.1007/s00216-014-8296-4
文献子类Article
英文摘要Cortex Lycii, the root bark of Lycium chinense Mill. or Lycium barbarum L., is a frequently used traditional Chinese medicine. Phytochemical studies have shown that phenolic amides are not only characteristic compounds but also abundant ones in this plant. In the present study, an effective method was developed for structural characterization of phenolic amides from Cortex Lycii by ultra-high performance liquid chromatography coupled with linear ion trap Orbitrap tandem mass spectrometry. The fragmentation of 14 compounds including six cinnamic acid amides, six neolignanamides, and two lignanamides were studied systematically for the first time. It was found that, in the positive ion mode, neutral loss of the tyramide moiety (137 Da) or N-(4-aminobutyl)acetamide moiety (130 Da) were characteristic for these compounds. At least 54 phenolic amides were detected in the extract and 48 of them were characterized, among which 14 known compounds were identified unambiguously by comparing the retention time and mass spectra with those of reference compounds, and 34 components were tentatively identified based on the fragmentation patterns, exact mass, UV spectra, as well as retention time. Fifteen compounds were characterized as potential new ones. Additionally, the developed method was applied to analyze eight batches of samples collected from the northwest of China, and it was found that cinnamic acid amides were the main type of phenolic amides in Cortex Lycii. In conclusion, the identification of these chemicals provided essential data for further phytochemical studies, metabolites identification, and the quality control of Cortex Lycii.
WOS关键词ACID-AMIDES ; ROOT BARK ; LIGNANAMIDES ; CHINENSE ; CONSTITUENTS ; PLANTS ; ALKALOIDS ; PEPTIDES ; TUBERS ; STEMS
资助项目National Natural Science Foundation of China[81373965] ; Twelfth Five-Year National Science & Technology Support Program[2012BAI29B06] ; Chinese Academy of Sciences[KSCX2-YWR-166] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing program" of the People's Republic of China[2011ZX09307-002-03]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000348207200022
出版者SPRINGER HEIDELBERG
源URL[http://119.78.100.183/handle/2S10ELR8/276766]  
专题上海中药现代化研究中心
通讯作者Yang, Min
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai 201203, Peoples R China;
2.ARS, USDA, Beltsville Human Nutr Res Ctr, Food Composit & Methods Dev Lab, Beltsville, MD 20705 USA;
3.ThermoFisher Sci China Co Ltd, Shanghai 201206, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Jingxian,Guan, Shuhong,Sun, Jianghao,et al. Characterization and profiling of phenolic amides from Cortex Lycii by ultra-high performance liquid chromatography coupled with LTQ-Orbitrap mass spectrometry[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2015,407(2):581-595.
APA Zhang, Jingxian.,Guan, Shuhong.,Sun, Jianghao.,Liu, Tian.,Chen, Pei.,...&Guo, De-an.(2015).Characterization and profiling of phenolic amides from Cortex Lycii by ultra-high performance liquid chromatography coupled with LTQ-Orbitrap mass spectrometry.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,407(2),581-595.
MLA Zhang, Jingxian,et al."Characterization and profiling of phenolic amides from Cortex Lycii by ultra-high performance liquid chromatography coupled with LTQ-Orbitrap mass spectrometry".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 407.2(2015):581-595.

入库方式： OAI收割

来源：上海药物研究所

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